Hi Monika,
Philippe is right, ITC can detect only binding with -dH > ~0.3 kcal/mole
(depends on instrument). If the binding is Entropy driven, i.e. TdS >> -dH,
than Kd might be quite low, like 20 uM, but ITC would still not detect it (this
often happens in mutants). You can try to optimize expe
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Hi,
Just to emphasize Philippe's statement: binding carbohydrates by proteins (lectins or other types) is often, if not exclusively, an entropy driven process. Hence, the low enthalpy contribution that you observe may be real. Maybe. My 2p thoughts.
Stay safe.
Boaz
