Yes
It requires electroporation and very careful handling of the bacmid. Other
than that it is a fairly simple process.
Here is an example reference:
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5067234/
Artem
On Sat, Jul 18, 2020, 9:12 PM Digant Nayak wrote:
> Dear all,
>
> Sorry for the o
Dear all,
Sorry for the off topic question, but i assure you that the long term
output of the project has structural biology application. I want to
transform a bacmid into E.coli cell and I was intrigued to find that there
is no protocol for this available on the internet (i would really
appreciat
I re-read this thread because I was confused by it, esp. the discussion on
having to use the command line to run old programs! I assumed therefore that
the old ccp4i gui had finally disappeared. Anyhow, I just installed 7.1 and
it's still there, complete with the Program List dropdown! In case a
I'm writing to acknowledge the passing of Ward Smith during the weekend of 5
July. Ward got his PhD with Martha Ludwig at U. of Michigan, and then came to
UCLA in 1977 to join Dave Eisenberg’s group as a postdoc. During the course of
things, he met Cheryl Janson, a Paul Boyer postdoc, and they w
