Re: [ccp4bb] Looking for method to find similar oligomeric arrangement

2020-06-20 Thread Boaz Shaanan
Hi, You can try the SSM from the pdbe tools, also described in Krissinel and Henrick (2004), Acta D63, 366-380. It worked for us nicely on a similar problem of a dimeric structure. Cheers, Boaz. Boaz Shaanan, Ph.D. Department of Life Sciences Ben Gurion University of the Negev Beer Sheva Israe

[ccp4bb] Looking for method to find similar oligomeric arrangement

2020-06-20 Thread Andrew Lovering
Dear colleagues, I have a structure of a simple, common fold (hth, dna-binding) that I believe has oligomerized in a different way to that observed for any members of the superfamily that I can reasonably analyze. So if I run fold comparison analysis (e.g. DALI) it will find similar structur

[ccp4bb] Amphipols for soluble protein stability

2020-06-20 Thread Srinivasan Rengachari
Dear all, I am working with a multi-subunit complex which needs a minimum of 5% glycerol for stability in solution. I usually reconstitute this complex together with multiple interaction factors as a part of big ensemble over GraFix for cryoEM grid preparation in a final buffer co