Yes - the “Fobs” is extracted from the twinned ”Iobs”
Which is actually I1 + I2*twin_factor , and yes, how to do that properly is
tricky.
On Tue, 28 Apr 2020 at 22:20, Frank von Delft
wrote:
> Hi all - feel free to point me to the docs if it's already clear somewhere:
>
> When refmac generates 2
Thank you
Artem
On Tue, Apr 28, 2020, 7:22 PM Thomas Cleveland
wrote:
> Hi,
>
> There are tons of these listed on eBay, many in very good condition or
> even unused. I used to get them there all the time.
>
> Best
>
> On Tue, Apr 28, 2020 at 8:51 AM Artem Evdokimov
> wrote:
>
>> CCP4 friends,
Hi,
There are tons of these listed on eBay, many in very good condition or even
unused. I used to get them there all the time.
Best
On Tue, Apr 28, 2020 at 8:51 AM Artem Evdokimov
wrote:
> CCP4 friends,
>
> Sorry for the somewhat off-topic post!
>
> For many years I've been a fan of the Pharma
Thank you very much!
Artem
On Tue, Apr 28, 2020, 1:03 PM Guenter Fritz <
guenter.fritz.phenix.c...@gmail.com> wrote:
> Dear Artem,
>
> I am a fan of the former "Superformance" column from Merck. The production
> / selling of these columns is sourced out to a small company called
> Goetec. The c
Hi all - feel free to point me to the docs if it's already clear somewhere:
When refmac generates 2mFo-DFc maps after twinned refinement, are they
the "untwinned" view of the electron density? I.e. with the twinnig
convoluted out by his statistical magic?
I remember Garib mentioning the ambi
A postdoctoral position is available in Dr. Yang Gao’s lab
(http://yanggaolab.blogs.rice.edu/) at Rice University, Houston. The Gao lab
utilizes structural biology techniques to investigate the molecular mechanisms
of DNA replication and repair. We employ cryo-electron microscopy, x-ray
crystal
Hello, it depends a bit on the resolution. With d<1.2 A you can, of course, do some quite complicated modelling of alternate confirmations, group occupancies, etc - actually you can do this very meaningfully at worse resolutions, too. My favourite subject - if your resolution is better than ~1.4 to
Dear Artem,
I am a fan of the former "Superformance" column from Merck. The
production / selling of these columns is sourced out to a small company
called Goetec. The columns are not really cheap, but good quality, last
forever, suited also for higher pressures, only glas and Teflon in
conta
Dear CCP4 community,
I would like to announce the following post-doctoral position to be
undertaken in collaboration with my research group in Campinas, São Paulo,
Brazil.
It would be a great opportunity for motivated structural biologists with
experience in medicinal chemistry.
Please find
I'm not aware that anything has changed since the last time this was
discussed on the BB. Currently there is no ideal solution since even
the mmCIF PDB format does not allow a proper description of the
situation of atoms whose position cannot be placed due to the absence of
data.
The "Ligan
Hello all,
This is one of those issues that seems to come up now and then. I have been
working on a structure that obviously has some radiation damage as indicted by
negative density and/or high thermal parameters. Since we know that residue X
is in the sequence the sidechain should be there
Dear Colleagues,
A full-time research lab technician position is available at the Michael
DeGroote Institute for Infectious Disease Research at McMaster University,
in Ontario, Canada. The position will be shared between the McMaster
Macromolecular Crystallography Facility and the Centre for Mi
I am locked down and dont have access easily to the lsqkab code. Remind me
- if/when we are able to go to the University again! to check this..
I am sure you are right - lsqkab certainly predates TER records..
All the best Eleanor
On Tue, 28 Apr 2020 at 14:18, benjamin bax wrote:
>
> Hi Eleanor,
Hi Eleanor,
Thanks for fix.
Sometimes I just superpose three atoms (making sure they are not in a straight
line).
But my atom superposition lsqkab script (below) - suggests that lsqkab has a
different atom count than used ‘standardly’.
Has anyone else found this problematic?
Thanks, Be
Omnifit are pretty good, I have packed a few of these. They have a good psi
rating and various fixed / adaptable end pieces for differing bed heights
https://kinesis.co.uk/knowledgebase/omnifit-chromatography-columns
From: CCP4 bulletin board On Behalf Of Artem Evdokimov
Sent: 28 April 2020 13:5
CCP4 friends,
Sorry for the somewhat off-topic post!
For many years I've been a fan of the Pharmacia XK columns and heavily
relied on them for the bulk of my work. Lately, however, GE has increased
the prices so much that I am reluctant to buy new columns from them. And I
don't mean filled column
I MEANT to upgrade lsqkab to accept DNA, and there is a small possibility
that I did!
Cheers Eleanor
On Tue, 28 Apr 2020 at 11:52, Carter, Charlie wrote:
> In my experience, lsqkab wouldn’t orient nucleic acid atoms, and I think
> Eleanor once told me I needed a different alternative for nucleic
In my experience, lsqkab wouldn’t orient nucleic acid atoms, and I think
Eleanor once told me I needed a different alternative for nucleic acids. If
this is no longer true, I’m happy to learn of it.
Charlie
On Apr 28, 2020, at 6:40 AM, benjamin bax
mailto:ben.d.v@gmail.com>> wrote:
HI Fre
HI Fred,
I still use command line version of lsqkab to do this kind of DNA fitting -
script below only uses mainchain atoms (not bases) which helps if you have
different DNAs.
Chain E and F are DNA.
Ben
./lsq-hinge-6fqv-bin-EV-B.com > lsq-hinge-6fqv-bin-EV-B.log
cat lsq-hinge-6fqv-b
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