Dear colleagues,
I wonder if there were a bit less controversial possibility. No matter if that
was less efficient. Would there be an option of using human cell lines that do
not origin from killed children? As far as I know, the HEK cells do. Sometimes,
the science can be pretty cruel.
I am sor
Hi Gloria,
Another vote here for HEK 293 Expi or Freestyle.
The off-the-shelf transfection reagents are super expensive, but I make my own
(happy to share protocols with anyone interested) and we normally get a few mgs
of protein per litre of suspension culture -- certainly enough for
crystall
In our lab, we see Expi293F works very good.
Thanks,
Reza
Md. Rezaul Karim
Graduate student, PhD Program in Integrated Biomedical SciencesMorsani College
of Medicine
University of South Florida
E-mail: reza...@yahoo.com, rez...@health.usf.eduPhone: +1-954-937-8487
On Friday, January 24, 202
Hello CCP4-ers,
I was wondering what people have found to be the best human cell line
expression system for making a large quantity of purified recombinant
protein.
Any information and protocols would be greatly appreciated.
Happy 2020, Gloria
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Dear CCP4 users,
The cilium organelle is constructed and maintained by the intraflagellar
transport machinery (see PMID: 30940671, 29658880, 26912722, 23990561). As
part of an International Training Network to study 'Ciliary Signalling in
Development and Disease', a PhD position to work on the str
*Postdoc position in protein biochemistry and crystallography*
We are looking for a highly motivated Postdoctoral researcher for our
protein biochemistry and crystallography laboratory at the Dept. of
Biochemistry at University Bayreuth, Germany. We use protein
crystallography and a variety o
Dear all,
We are happy to inform you that the Call for Regular Proposals for PETRA III
beamline P11 is now open.
This call relates to beamtime during July-December 2020.
The deadline for PETRA III proposal submission is
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