Hi Nestor,
I think the reason for Arg side chain to curl up is because I refined
ligand and Arg side chain with occupancy refinement, and the Arg moved
away from the density, most likely due to 'repulsion' from ligand.
The other question is: Is it possible that the H-bonds stay very
close
Second Notice:
Dear all,
We are glad to announce the BioSAXS2019, a workshop on biological
small-angle X-ray scattering at Stanford Synchrotron Radiation
Lightsource (SSRL).
When: December 2-5, 2019
Where: SSRL, SLAC National Accelerator Laboratory, Menlo Park, CA
The SSRL Structural Molecu
Reminder One week to London CCP4i2 Road show. Places still available.
*CCP4 i2 Road Show, London*
There will be a CCP4 Road Show for use of the i2 interface on Tuesday
12th November, at Birkbeck College.
*Time:* 14:00 - 16:00 for the main workshop with about an hour
afterwards for those wi
It happens.
Ef-Tu is methylated on a single lysine
Also LafV is a putative methylase in E. Coli but its effects were not
studied in detail.
Salmonella methylates flagellin which is how lysine methylation was
discovered - and E.coli is a kissing cousin so...
Artem
On Tue, Nov 5, 2019, 12:31 Je
Dear CCP4 community,
I am hoping that someone here might know the answer to this as I cannot
find anything concrete in my literature search.
I have recently received a mass spec result indicating that a
recombinant protein expressed in /E. coli/ is heavily lysine methylated.
I would like to
Dear HK,
If you believe that R120 only adopts one conformation then you should not
refine the occupancies of the side chain atoms. It makes no physical sense to
have occupancies less than one for these atoms, but occupancy equal to one for
the rest of the protein (unless you believe the side ch
Dear colleagues,
We are currently looking for a postdoctoral researcher to join our lab.
Please see below for details:
*OFFER* – Postdoctoral Position (Two years)
*DEADLINE: *4th of February, 2020 at 17:00 h CET or until position is
filled.
*WHERE* – Basque Centre for Biophysics (Bilbao
Dear HK,
No that's not quite correct - 'occupancy group alts complete' means that both
R120 and the ligand are constrained so that their occupancies sum to unity. In
contrast, 'occupancy group alts incomplete' means that the occupancies of R120
and the ligand will not be constrained to sum to u
Dear all,
I have problem with occupancy refinement by Refmac5 for an overlapping electron
density of part of residue (an arginine) and part of ligand (tetracyclic
compound) (attached figures in Dropbox with a link as shown below).
https://www.dropbox.com/sh/ppmfp5dnpy1b9e9/AAAV79bOzPHQUrVYp9loM
On 05/11/2019 09:53, Fuentes-Montero, Luis (DLSLtd,RAL,LSCI) wrote:
Hi FH,
It looks to me like an installation issue, I am CCing you to the rest of the CCP4BB with hope to find anyone
who knows what to do:
Guys: Does anybody faced the following error message before?
*undefined symbol: PyUnic
Hi FH,
It looks to me like an installation issue, I am CCing you to the rest of the
CCP4BB with hope to find anyone who knows what to do:
Guys: Does anybody faced the following error message before?
undefined symbol: PyUnicodeUCS2_DecodeUTF8
Thanks in advance,
Luiso.
_
Hi Pavel
I work with peptide-scaffold chimeras on a daily basis. Initially, I tried to
include the scaffolds as HETATM and use JLigand to create the links between the
scaffolds and the natural amino acids. This procedure however is rather fussy
and by now I build the chimera in silico, using na
Hi Michael,
I believe you are looking at the anisotropic B factor. 8 A2 is very low,
you are all good.
The Wilson B is given by Truncate if I'm not mistaking.
Best regards
Vincent
Le 04/11/2019 à 00:18, Michael Jarva a écrit :
Hi CCP4BB,
I have some unusual crystal diffraction data I'd like
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