Dear Michael,
Exactly because the B factor is only 8, the intensity as function of resolution
is droping really slowly, and thus the I/sigI 4.7 at low resolution drops so
slowly to 1.0.
I would almost bet you have a low solvent content.
The data are fine imo.
Best,
Tassos
On Nov 4, 2019, at
The Phenix developers are pleased to announce that version 1.17 of Phenix is
now available (build 1.17.1-3660). Binary installers for Linux, Mac OSX, and
Windows platforms, and the source installer, are available at the download site:
http://phenix-online.org/download/
Please note that
Given that the I/sigma(I) is quite low the high R-merge values are probably to be expected. If the data were stronger, an R-merge that high would indicate that the space group was wrong or the data misindexed. If the multiplicity was higher you could process the data in lower symmetry space group (
Hi Jacob
Not the easiest task... Based on past experience your major issue will be
the incredible abundance of sodium ions in everything.
So assuming you have high quality sodium free solutions and are willing to
work exclusively in plastic, quartz or fused silica - here are a few
thoughts:
1. N
Dear Crystallographers,
Does anyone know of a good biophysical way to identify or quantify sodium ion
binding to a protein, besides crystallography and ITC? Is this possible with
SPR, perhaps? Mass spec? Gel shifts? Examples would be greatly appreciated!
All the best,
Jacob Keller
++
Hi CCP4BB,
I have some unusual crystal diffraction data I'd like to get your input on.
Almost a year ago I shot some small rods sticking out of a loop, so basically
no liquid around them - using the microfocus MX2 beamline at the australian
synchrotron, collected on an EIGER 16M detector.
The
Dear All,
On behalf of the organizing committee, we would like to invite you and your
group members to participate at the International Symposium on Cell Surface
Macromolecules to be held in IISER and NCL Pune, India from Feb 17-20, 2020.
This meeting is part of a series held once in 3 years to b
