Re: [ccp4bb] design specs/tolerances for crystallization rooms?

2019-09-24 Thread Frank Von Delft
My colleague Opher Gileadi gave us an excellent tip when we were designing our 4C harvesting room, over a decade ago: set it to 7C. The crystals are unlikely to mind, but it's SO much more comfortable to be in for hours. I seem to remember he mentioned something like a comfort inflection point

[ccp4bb] design specs/tolerances for crystallization rooms?

2019-09-24 Thread Scott, Emily
Anyone out there specifically design rooms for (protein) crystallization at ~22 deg and 4 deg C? If you have successes or failures and can share any design specs with regard to vibration, temperature, and humidity tolerances, it would be much appreciated to pass on to the architects for our new

[ccp4bb] Beam time available at upgraded MacCHESS

2019-09-24 Thread D. Marian Szebenyi
Got crystals to check out? The Fall 2019 run at CHESS begins October 16, and it's not too late to apply for time. Proposals are accepted any time for beam time at the newly upgraded MacCHESS beamlines: BioSAXS station (formerly G1) - on-site or mail-in BioSAXS, featuring: * 7-14 KeV, standard b

Re: [ccp4bb] Are there any proteins capable of crystallizing at a wide range of pH having the same space group?

2019-09-24 Thread Murpholino Peligro
I just want add that the redundant PDB got a new site http://pdbflex.org/ (It's pretty cool actually) Thanks to Robyn for pointing that out. El dom., 22 de sep. de 2019 a la(s) 10:18, Robyn Stanfield ( ro...@scripps.edu) escribió: > Hi, > > The Burnham changed names sometime back, that may be w

Re: [ccp4bb] Optimisation of 2D very thin plates to thick plates

2019-09-24 Thread Sabine Buschmann
Dear Will, if the crystals grow in vapour-diffusion, you can try to overlay the reservoir with oil to slow down the crystallization process. I know at least two cases where this technique helped to get plate-shaped crystals into more 3D shape. http://scripts.iucr.org/cgi-bin/paper?S00218898960135

[ccp4bb] Cryo-EM sample preparation workshop at the ESRF / 5th to 7th of November 2019 (last week before closure of registration)

2019-09-24 Thread David FLOT
Dear All, We are pleased to announce a 2.5 day practical winter workshop on sample preparation and negative staining for cryo-EM single particle data collection jointly organised by the ESRF, IBS, ILL and the EMBL Grenoble. This is the third of a series of practical hands-on workshops started