I totally agree. And because the new dataset is of higher resolution, there
will be new reflections in the test set anyway.
Now if you want to use the same testset always for series of isomorphous
datasets (even though you do not need to), you can do this:
- Use unique to make a full set of refl
Dear Mariana,
you can simply create a new set for Rfree, independently of the previous one.
It will be as good as the copied one, and there is no reason why you would
need to maintain the same flags. As Ian Tickle explained only briefly ago,
your new data set will have new, independent errors.
You should be able to get an idea of whether it has worked or not with viewHKL
in the old GUI e.g. find a few reflections with R-free flag zero in your old
dataset and note the indices. Then you can search the new dataset for these
reflections and see if they are still flagged zero. You may nee
Dear all,
We tried to use the Rfree set from a lower resolution data with a higher
resolution from the same Crystal. To do so We used aimless at ccp4i with the
option use free flag from another mtz file and extend the data.
I think it worked, but now we don't know how to be sure we have the s
Dear All,
The last date for registration for the *EMBO Practical course
CEM3DIP 2020: Single particle cryoEM of macromolecular-assemblies and
cellular tomography*
has been extended to *25 Sep 2019.*
*To register for the course, please click here:*
http://meetings.embo.org/event/20-cem3di
Dear James,
Here you go, a “grand challenge” suggestion to consider for funding from the
“James Holton Foundation for structural biology research”:-
“The human genome/proteome in 3-D”
Greetings,
John
Emeritus Professor John R Helliwell DSc
> On 14 Sep 2019, at 02:39, James Holton wrote:
>
>
