I would back way up in the process and verify that the basics are correct.
Ten molecules in the ASU is unusual unless your molecules are forming
defined oligomers, e.g., 2.5 tetramers, etc. Precise predictions of
monomers in the ASU by Matthews number is increasingly unreliable above 2-3
monomers.
Sorry for the initial message. I tried to attach Matthews coefficient
calculation figure but failed to do so, which resulted the message as not plain
text. Below is my question, thanks.
I collected one dataset and processed it to 3.6 angstrom. my protein is quite
small with only 14 kDa. It is
Dear all
I collected one dataset and processed it to 3.6 angstrom. my protein is quite
small with only 14 kDa. It is estimated over ten molecules in one ASU based on
Matthew coefficient calculation.
However, only ten molecules can be correctly placed with good fitting. I can
observe extra Fo-F
Dear All,
The Multiscale Research Institute for Complex Systems (MRICS) at Fudan
University is located at the Zhangjiang Campus of Fudan University and is
supported by the Shanghai High-level Talents Program.MRICS is strongly
committed to the development of noveland effective multi-scale imaging
Hi Joe,
I recently submitted 4 structures to the PDB, and I found that an ORCID was
necessary for the corresponding author. However, additional authors were
added without an ORCID.
Hope this is somewhat helpful.
Best,
Crissy
On Mon, Aug 19, 2019 at 2:55 PM Jie Liu wrote:
> Dear all,
>
> It's
Hello,
like a lot of items in the pdb entry, the entry is not mandatory. But using the
ORCID is a good idea in order to be able to claim easily your work if you have
a very common name and it may be difficult to find your authorship
unambigously.
Best
Wim
De: "Jie Liu"
À: "CCP4BB"
Envo
Dear Jie
ORCiD is mandatory for all authors that we communicate with during
deposition (contact authors). One of the reasons is to allow us ensure that
we are able to associate contact authors with all of their depositions even
when their email address changes.
These contact details are kept
Dear all,
It's been a while since last time I deposited structures to PDB. Do I really
need an ORCID (Open Researcher and Contributor IDentifier) now to submit files?
Is it mandatory?
Thank you!
Jie
To unsubscribe from t
Dear All,
Pymol seems to ignore the HELIX and SHEET records in my coordinate file,
and instead assigns secondary structural features according to the default
method. Unless my understanding is flawed, these records should override
Pymol's assignment...?
I generated the records with the Stride Web
Dear Chandra,
where at APS did you collect? NE-CAT (and in particular beamline 24-ID-E)
is well set-up for long unit cells. You could ask Yury Polikanov (
https://bios.uic.edu/profiles/polikanov-yury/) for advice. He crystallizes
ribosomes with unit cell that are not quite as big as what you ha
I would respectfully suggest that higher pixel resolution does not generally help much
in these situations. If an average spot is 10 or more pixels wide then the profile is
defined pretty well. But if the spots overlap, they still overlap with higher pixel
density. It may make profile fitting
Chandra
What you are looking for here is a beamline with a detector with many pixels
(so you can resolve the long axis) and a multi-axis goniometer - probably a
SmarGon / kappa and an Eiger 16M would make a good combination for this.
Searching on
http://biosync.sbkb.org/
Should allow you to m
Dear All
We recently collected a data set at APS, Chicago with unit cell dimensions of
68.4; 68.4 and 991.6 A. Our diffraction data extends to 3A with the APS set up,
however, the long axis has been problematic, resulting in streaking of the
diffraction data and requires a very specific orienta
Dear Colleagues,
An exciting research opportunity is available in my team:
Postdoctoral position in Membrane Protein Structural Biology and Biophysics at
The Pennsylvania State University College of Medicine
The laboratory of Dr. Kenneth Lee seeks applications for a postdoctoral
position apply
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