Re: [ccp4bb] RDRP crystal

I am not sure if you have tried seeding as well. That helped me in growing bigger crystals. Best, Bharti Singal [http://www3.ntu.edu.sg/exchange/outlook2007/composeandsendmsg/ntulogofit2.gif] Bharti Singal | PhD Student | Division of Structural Biology & Biochemistry | Nanyang Technological Un

Re: [ccp4bb] RDRP crystal

Hi Zhangyi, You can try using capillary counter diffusion method. Not sure if it can help, but you can give it a try. You can check the following paper. https://www-sciencedirect-com.ezlibproxy1.ntu.edu.sg/science/article/pii/S1047847703000522?via%3Dihub#FIG2 Best, Bharti Singal [http://www3.n

[ccp4bb] RDRP crystal

Dear all, A single positive stranded RNA virus RDRP monomer grows crystals, precipitants (PEG8000) with low concentration, more nucleuses and poor resolution.Reduce precipitant concentration can reduce the crystal nucleus, but worse resolution, precipitant concentration increased, many crystal n

[ccp4bb] image

Dear all, A single plus-stranded RNA virus RDRP monomer grows crystals, precipitants (PEG8000) with low concentration, more nucleuses and poor resolution.Reduce precipitant concentration can reduce the crystal nucleus, but worse resolution, precipitant concentration increased, many crystal nucle

Re: [ccp4bb] crystal contacts and biologically relevant contacts

Hi Alex, Yes this is acceptable - symmetry-related proteins are identical and interchangeable. It can be helpful to use the PISA program (a plugin for Coot or as a webserver http://www.ebi.ac.uk/msd-srv/prot_int/cgi-bin/piserver) to identify which symmetry-related proteins are forming the biologica

[ccp4bb] crystal contacts and biologically relevant contacts

Dear Colleagues, Recently I solved one Ab-Ag complex structure. It was indexed as p212121 or p21212 space group and solved the structure with one Fab and one Ag in the cell unit. The contact interface is not the one I expected (elbow region contacts with Ag). Using symexp I got several symmetry re

[ccp4bb] New PDBe-KB aggregated views of protein structure

*New PDBe-KB aggregated views of protein structure* We are delighted to announce the newprotein-centric aggregated views , developed as part of our new collaborative resourceProtein Data Bank in Europe Knowledge Base (PDBe-KB)

Re: [ccp4bb] map rotation

This can do done, but I suspect it is much easier using COOT tools - then you can output a map from COOT. Will check later .. Eleanor On Thu, 21 Mar 2019 at 07:28, Zhijie Li wrote: > Hi Jan, > > > Sorry I didn't read your script earlier. If you change your mapmask > command to output a map inste

Re: [ccp4bb] map rotation

Hi Jan, Sorry I didn't read your script earlier. If you change your mapmask command to output a map instead of a mask it may work for you: mapmask \ mapin 2mol_2mFo-DFc.map \ xyzin 2mol_B.pdb \ MAPOUT B.map \ << eof border 2 eof then you should get a map that covers the whole molecule