[ccp4bb] AUC2019 – registrations are open.

2019-03-06 Thread Renwick Dobson
Dear Structural Biology community, AUC2019, the 24th AUC Workshop and Symposium, will be held at the University of Canterbury, Christchurch (New Zealand) from 24-29 August 2019. The format of the event is a 2-3 day workshop offer

Re: [ccp4bb] off topic: Membrane protein "into" soluble protein

2019-03-06 Thread Bonsor, Daniel
In theory yes, you can fuse the termini to restrain the protein. You could use T4 lysozyme and insert in the loop which they use for crystallizing GPCRs (many references, including https://www.ncbi.nlm.nih.gov/pubmed/25450769). The potential problems are that you have no idea where the termini a

Re: [ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

2019-03-06 Thread Phoebe A. Rice
Slightly off-topic, and I may have recommended these some time ago on the BB (sorry), but we find these toy turtles very useful for explaining symmetry, particularly screw axes which some students have a really hard time visualizing from 2D diagrams: https://www.amazon.com/Fat-Brain-Toys-FA042-

[ccp4bb] ISSC19 Second Announcement

2019-03-06 Thread Brehm, Wolfgang
Second announcement 4th International Summer School of Crystallography, June 2 - June 8, at the Centre for Free-Electron Laser Science at DESY, Hamburg. Detailed information can be found on the school web page: https://conferences.cfel.de/issc19/ Application deadline: May 1, 2019 Prof. Carmel

[ccp4bb] off topic: Membrane protein "into" soluble protein

2019-03-06 Thread Andrew Lovering
Dear ccp4bb members, A quick off topic question. I have a protein whose domain structure runs N-TM-receptor-TM-enzyme-C, i.e. is a two transmembrane-helix containing signalling protein. One would suspect that the receptor domain has ends that cluster, and that the enzyme (via homology with know

Re: [ccp4bb] Question about the electron density maps (.ccp4) in PDBe

2019-03-06 Thread Yao, Sen
Thanks for the clarification, Pavel! And the article is very helpful as well. Cheers, Sen On Tue, Mar 5, 2019 at 1:07 PM Pavel Afonine wrote: > P.S.: while on the topic, it might be helpful to you to have a look at > this article ("On the analysis of residual density distributions on an > absol