[ccp4bb] Crystallography position in New York City

Dear CCP4BBers, We have a position available for a collaborative project between the labs of David Baker (https://www.bakerlab.org ) and Gira Bhabha & Damian Ekiert (http://bhabhaekiertlab.org ). The position will be based in New York City

Re: [ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

Dear Pietro I have used many different gratings for the purpose over many years... Ones that I found very rewarding are writeable CDs and DVDs. They often come in a pack "protected" by a empty CD / DVD matrix hat is only plastic with grooves and no silver (or whatever the shiny recording material

[ccp4bb] Postdoctoral Position in Structural and Functional Studies of Telomeric Complexes

A Post Doctoral Fellow position is immediately available in the laboratory of Dr. Emmanuel Skordalakes at The Wistar Institute. The laboratory studies protein, nucleic acid assemblies that participate in the replication and maintenance of telomeres and have been implicated in a host of diseases

[ccp4bb] Postdoctoral position at the University of Chicago

The Mendoza group at the University of Chicago is looking to recruit highly motivated, productive, and challenge seeking individuals. The Mendoza group applies protein engineering strategies to solve challenging complex structures (Mendoza et al., Nature, 2019; Mendoza et al., Immunity, 2019)

[ccp4bb] Software Engineer position at Diamond Light Source

Dear Colleagues If you have experience of software development using Python or Java and a keen interest in using your skills to enable experiments across a unique range of MX beamlines (https://www.diamond.ac.uk/Instruments/Mx) please consider applying for the Senior Software Engineer position

Re: [ccp4bb] Question about the electron density maps (.ccp4) in PDBe

P.S.: while on the topic, it might be helpful to you to have a look at this article ("On the analysis of residual density distributions on an absolute scale", page 43) available here: http://phenix-online.org/newsletter/CCN_2012_07.pdf Pavel On Tue, Mar 5, 2019 at 10:02 AM Pavel Afonine wrote:

Re: [ccp4bb] Question about the electron density maps (.ccp4) in PDBe

Hi Sen, As Pavel mentioned, phenix.f000 will give you F(0,0,0) value, but I don't > see that information stored and easily calculated from .ccp4 data. > phenix.f000 requires atomic model (PDB or mmCIF file) as input, not a map. If you want bulk-solvent to be added, then you need to give it mean

[ccp4bb] 2019 PSFaM meeting

To ccp4 community, Please distribute invitation to the 7th Annual Meeting in Protein Structure, Function and Malfunction (PSFaM) organized by College of Medicine, University of Saskatchewan. June 20-21, 2019, Saskatoon. link: http://cmcf.lightsource.ca/psfam/ registration is free. Sincerely,

Re: [ccp4bb] Question about the electron density maps (.ccp4) in PDBe

Dear Sen, Our messages just crossed ... . With best wishes, Gerard. -- On Tue, Mar 05, 2019 at 11:32:01AM -0500, Yao, Sen wrote: > Dear Gerard, > > Thank you for your reply. I totally agree with you. > > The average value of the 2mFo-DFc map should be zero, and from my > ob

Re: [ccp4bb] Question about the electron density maps (.ccp4) in PDBe

Dear Sen On Tue, Mar 05, 2019 at 11:05:26AM -0500, Yao, Sen wrote: > Thank you Ian! The response is really informative. > > I am aware that electron density is a point sample. And multiply it by the > voxel volume is an estimated average. I do have a normalization step in my > calculation that sh

Re: [ccp4bb] Question about the electron density maps (.ccp4) in PDBe

Dear Gerard, Thank you for your reply. I totally agree with you. The average value of the 2mFo-DFc map should be zero, and from my observation, most of them are a very small number (<0.001) that can be viewed as zero practically. As Pavel mentioned, phenix.f000 will give you F(0,0,0) value, but I

Re: [ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

Dear Pietro, I'am using the slides from the ICE: https://icestore.chem.wisc.edu/product/optical-transform-kit Cheers, Eckhard Am 05.03.2019 um 10:41 schrieb Roversi, Pietro (Dr.): Dear all, I'd like to purchase slides with diffraction gratings for educational purposes. Ideally, to be used wi

[ccp4bb] PhD position in structural biology of membrane transporters - Imperial College London and Diamond Light Source

Dear All, we have an exciting 3-year PhD studentship between Imperial College London (Beis lab>) and Diamond Light Source (Wagner lab

Re: [ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

Thank you Philippe, this sound wonderful, I cannot download the PDF (dangling link ) - would you be able to let me have it? THanks P Pietro Roversi LISCB Wellcome Trust ISSF Fellow https://bit.ly/2I4Wm5Z Leicester Institute of Structural and Chemical Biology Department of Molecular and Ce

Re: [ccp4bb] Change dimer assembly in ASU

I tend to use PISA for suggesting biologically relevant complexes. And you can modify the MR solution to replace a molecule with any symmetry generated copy of it.. But check Phaser has put your molecule reasonably close to the origin. On Mon, 4 Mar 2019 at 19:52, Randy Read wrote: > Dear E

Re: [ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

Le Mardi 5 Mars 2019 10:41 CET, "Roversi, Pietro (Dr.)" a écrit: Many years ago I did teaching on diffraction by illustrating the major aspects of crystal structure determination with 2D crystals on 24x36 films. The crystal was an 80x60 repeats of the famous Einstein's tongue and MIR was illu

Re: [ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

Dear Pietro the ones that I have just used in my lecture are from https://www.patonhawksley.com best wishes Savvas > On 5 Mar 2019, at 10:41, Roversi, Pietro (Dr.) wrote: > > Dear all, > > I'd like to purchase slides with diffraction gratings for educational

Re: [ccp4bb] Electron scattering factors for SHELXL

Dear Matthew, For most of my work with electron diffraction, I used SHELXL for refinement (e.g. https://doi.org/10.1002/anie.201811318, https://doi.org/10.1107/ S2052252518000945, or http://www.dx.doi.org/10.1107/S2053273315022500). L Peng ("Electron atomic scattering factors and scattering pote

[ccp4bb] Purchasing slides with diffraction gratings for teaching diffraction

Dear all, I'd like to purchase slides with diffraction gratings for educational purposes. Ideally, to be used with a visible-light laser pointer. And: with 1D and 2D patterns, of variable repeats - so as to be able to illustrate the effect on the spacing in reciprocal space, as it were. I am l

[ccp4bb] Symposium "Macromolecules in Action” 4-5. July 2019, Grenoble

(on behalf of the organisers) Dear colleagues, This is to invite you to the Symposium “Macromolecules in Action” organised by the Partnership for Structural Biology (PSB) in Grenoble, France, on 4-5 July 2019. This meeting aims to illustrate how big biological questions can be resolved in