On 03/02/2019 09:59 PM, Ronald E. Stenkamp wrote:
I was taught about centric reflections using different words from those in the
wiki.
If you look at your crystal structure in projection and the planar view looks
centrosymmetric, the zone of reflections corresponding to that projection will
h
SDS is soluble, whereas the potassium salt of dodecyl sulphate is insoluble.
You could try 18-Crown-6 ether to chelate the potassium, though I don't know if
the crown ether would then affect the gel.
Dan
Daniel A. Bonsor PhD
Institute of Human Virology,
University of Maryland at Baltimore
725
Dear crystallographers,
It has been my experience that KCl does nasty things when loading SDS-PAGE
gels. Does anyone have an easy workaround, perhaps TCA precipitation? Ideally
this would be something nicely quantitative yet quick and easy….
Any suggestions appreciated.
All the best,
Jacob Ke
Hi Alex,
In my experience you just have to experiment with these parameters (in
however of a limited space that is afforded by your experimental set-up).
If you have the right tools you can slog through semifactorial or DoE-based
scouting of various parameters with relative ease. These parameters
Thaks! typo- h,0,l not h,0,k
I have not registered for editing on that wiki,
so I was hoping someone else would take care of it.
But it seems all that's needed is to confirm your account, so I'll register and
give it a try. Hope I don't make things worse!
(what is this urldefense.proofpoint? did
You are correct, other than your typo. The centric zone in a
monoclinic space group (B setting) is h0l.
This web site is a wiki so you should be able to correct it yourself.
Dale Tronrud
On 3/2/2019 2:00 PM, Edward A. Berry wrote:
> The wiki:
>
> https://strucbio.biologie.uni-konstanz.de
The wiki:
https://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Centric_and_acentric_reflections
says:
"A reflection is centric if there is a reciprocal space symmetry
operator which maps it onto itself (or rather its Friedel mate).
. . .
Centric reflections in space group P2 and P21 are
