Hi Nicola,
Our TurboTEV (http://accelagen.com/TurboTEV-datasheet.htm) is very stable and
does not need additional reducing agent for its activity. The storage buffer
has 1 mM TCEP. It'll be likely <0.1 mM in your digestion. You can get it from
our European distributors or directly from Accealge
Hi Kevin,
Since CCP4 v.7 there is an issue with the term Windows. I was used to run any
CCP4 program from anywhere on my disks with v. 6.x but with v.7x you have to
move every needed fiies to C:\CCP4\ccp4-7.0 (or something like this): Indeed
where the shortcut of the CCP4 consol is located.
So
Hi Nicola,
0.1-1mM Cysteine, GSH or BME will work fine. You can also try using very fresh
TEV without reducing reagent (or store it with BME and remove the reducing
reagent by some column just before use). Depending on how concentrated the
stock is, diluting it 3-5x could also sufficiently red
You can use a redox system which should maintain disulfide bonds, but still
offer enough reduction to give TEV active. Use 3mM reduced glutathione/0.3mM
oxidized glutathione. Add the protein to TEV so the DTT is rapidly diluted.
We started to make our own when it rapidly became used my nearly e
I am interested in purifying proteins with 1 or 2 disulphide bonds, and have
been using enterokinase to cleave off N-terminal tags but we have had issues
with poor cleavage and want to try TEV cleavage instead. However TEV protease
is usually kept in a high amount of DTT and I am concerned about
Dear ccp4bb enthusiasts,
Please help spread the word about the postdoc position described below.
Thank you,
Alexandra
*POSTDOCTORAL POSITION*
*at Brown University, USA*
The Deaconescu Lab at Brown University has one opening for a
postdoctoral researcher. The laboratory's interests lie prim
Dear Herman,
On Thu, Feb 07, 2019 at 10:26:09AM +, Herman Schreuder wrote:
> I did understand your question correctly and (at least for ligands)
> the procedure I and also Diana Tomchick described, worked. However,
> I just did a test with both Refmac and Buster and it seems that
> these progr
Dear Domen,
I bought a Lenovo Thinkpad p72 for both MX and cryoEM work last year and I
am generally happy. The Xeon CPUs do get hot from time to time when
governor is set to performance, but the thing is well ventilated and not
too noisy.
The Quadro GPU I have is, performance-wise, equivalent to
I've been really happy with the performance of my laptop (a 2016-era
Asus ROG Strix GL502vs) for MD work. It's a gaming model (GTX 1070
rather than Quadro, and an i7 CPU), but it's solid and seriously
powerful. I dual-boot Fedora and Windows (I initially tried with Ubuntu,
but its setup crashed
Dear CCP4 community,
My 8-year-old Dell Inspiron with Ubuntu 10 is waving goodbye and I would
ask for some help on this bulletin as buying/setting up a new workstation
is common trouble in our community.
Has anyone recently set up a laptop for structural work with all the MD,
docking, etc. *in sil
Hi! Can anyone run refmac from the WINDOWS command prompt?
I've been experimenting with various combinations of environment variables,
but I always get an error. The one instance of this error on google is an
unanswered CCP4BB question.
Dictionary path has not been defined
Check the environment v
Dear all,
There are still some places left for our two model building workshops. Please
note deadline for applications is tomorrow (14th Feb).
(1) Introduction to model building and refinement using cryo-EM maps.
14-15 March CNIO, Madrid
Introduction to atomic model building using EM data. Th
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