Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

Try EPPIC (http://eppic-web.org), it will give you all possible symmetric assemblies in your crystal and their constituent interfaces, listing the symmetry operators too. See some help here http://eppic-web.org/ewui/#help Jose On Sat, 2 Feb 2019 at 16:42, Ricardo Padua wrote: > Hi Erik, > > Hav

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

Hi Erik, Have you tried Achesym? http://achesym.ibch.poznan.pl Kowiel, M., Jaskolski, M. & Dauter, Z. (2014). ACHESYM: an algorithm and server for standardized placement of macromolecular models in the unit cell. Acta Cryst. D70, doi:10.1107/S1399004714024572. Best Ricardo Padua On Sat, Feb 2

Re: [ccp4bb] Generating rotation/translation matrices for biological assemblies

Hi Eric, You can lookup the symmetry operator in the International Tables (http://img.chem.ucl.ac.uk/sgp/LARGE/sgp.htm). Alternatively, you can use coot to find the crystallographic symmetry operator that generates the second half of your tetramer. Draw/Cell & Symmetry and then generate the sym

[ccp4bb] Generating rotation/translation matrices for biological assemblies

Hi all, I'm working on a protein that I believe is tetrameric based on AUC, gel filtration, and crystallography. However, although my asymmetric unit has 4 chains, I cannot form the tetramer within the asymmetric unit. Instead, the asymmetric unit has two half-tetramers ('dimers'), and each fu

Re: [ccp4bb] Crystallization problems using MPD (2-Methyl-2,4-pentanediol)

Hi js It happened to us as well with a 1:1 protein complex with decent affinity (Kd about 1 uM). The MPD-based condition (quite high % MPD if I recall correctly) would break the complex apart and only one of the two components formed a crystal (effectively allowing crystallisation of a sample w

Re: [ccp4bb] Crystallization problems using MPD (2-Methyl-2,4-pentanediol)

Hi, If the interaction between partners is of a hydrophobic nature and involves leucines, it may be MPD destroys the interaction. Have a look at the following publication: Acta Crystallogr D Biol Crystallogr. 2002 Oct;58(Pt 10 Pt 1):1722-8. Epu

Re: [ccp4bb] Is there any alternative to siliconized glass coverslips for crystallization?

Hanging or sitting drops, it's there any other reason you're looking for different cover slides? There are hydrophilic/phobic finishings to pick from, plus oil is also an alternative both to treat the surface or to screen in oil (micro)batch. Plus if hanging is your thing, dispense on a sittin