Re: [ccp4bb] AutoBuild Invalid MTZ column_types

2018-12-15 Thread Eleanor Dodson
Looks as though the column types have been run together.. Xia3 writers? It should read "F Q D Q" I believe.. Eleanor On Sat, 15 Dec 2018 at 15:56, Robson-Tull, Jacob < jacob.robson-tul...@imperial.ac.uk> wrote: > Dear all, > > > Has anyone encountered the error "Invalid MTZ column_types for the

Re: [ccp4bb] Non-specific disulfides in a secreted protein

2018-12-15 Thread Tomas Malinauskas
Dear Zhijie et al, thanks a lot for your thoughtful suggestions. a) we do get a fraction with a "proper" monomer but it would be nice to minimise losses because of non-specific disulfides; b) yes, it has 1 Asn-linked glycan which gets nicely trimmed upon treatment with Endo F1; d) yes, we do get so

[ccp4bb] AutoBuild Invalid MTZ column_types

2018-12-15 Thread Robson-Tull, Jacob
Dear all, Has anyone encountered the error "Invalid MTZ column_types for the given miller_array" when trying to run AutoBuild? The last line of the log was "Adding array ['F', 'SIGF', 'DANO', 'SIGDANO'] to output file with type FQDQ". The MTZ files were built with the Xia2 3dii pipeline. I

Re: [ccp4bb] Non-specific disulfides in a secreted protein

2018-12-15 Thread Luca Jovine
Dear Tomas, Along Radu’s comments, here’s another paper well worth looking into: Halff EF, Versteeg M, Brondijk TH, Huizinga EG When less becomes more: optimization of protein expression in HEK293-EBNA1 cells using plasmid titration - a case study for NLRs https://www.ncbi.nlm.nih.gov/p