[ccp4bb] Looking for alternatives for discontinued crystallography items

Hi, I am looking for alternative suppliers or similar items for the following products that were carried by Hampton Research but have since been discontinued. 1. Cryo vial holder. This is a hexagonal, metal holder that can hold 8 cryo vials. It is handy for freezing multiple crystals at once an

[ccp4bb] Cryo-EM Post-Doctoral Opportunities in New York

The Fitzpatrick lab, a member of the Zuckerman Mind Brain Behavior Institute at Columbia University, has 5 openings for postdoctoral research associates funded by a recent NIH U01 award to study the structural biology of tau and TDP-43 in neurodegenerative diseases. Situated in the heart of Ma

[ccp4bb] AW: [EXTERNAL] [ccp4bb] Assumptions on protein purification

Dear Markus, I believe the strong assumption in the community is that a clear single peak of appropriate Mw is a clear indication of pure protein, worthy intensive crystallization efforts. Whether it is active is another question and this should be measured. For your analysis, it is not import

Re: [ccp4bb] Weird diffraction pattern

Dear all Thanks for all the input both on- and off- the list. We shall definitely look into these suggestions further and report again here in due course. Kind regards Sam On Tue, 9 Oct 2018 at 19:12, Sam Tang wrote: > Dear all > > Hello. We recently shot a crystal (a protein with small mole

[ccp4bb] Assumptions on protein purification

Dear all, Not directly a ccp4 question. I am working on a multi-domain protein with multiple catalytic centres. Purifying in gel-filtration (Äkta) with different buffers gives a clear distinct peak indicating pure protein. We could even crystallise it and determine 3D structure to about 2.5 A. Why

Re: [ccp4bb] Issue with high Rfree (0.25) for a high-resolution dataset (1.05 Ang)

Dear Guto, integration programs often ignore overloads, and most of them know the detector cut-offs. mosflm has the option to estimate the resolution of overloaded reflections from the none-overloaded parts. This should not be a replacement for proper collection of a low resolution data set, but i

Re: [ccp4bb] Issue with high Rfree (0.25) for a high-resolution dataset (1.05 Ang)

Hi all, Firstly, I’d like to say I’m really impressed with the volume, speed and detail of the response that I have received. Thanks everyone. Overloads Eddie, Marc, Eleanor, Harry and Misha; thanks to all for suggesting collection strategies and identifying the likely issue. Yesterday, I had a