In my case what simply worked is to put your solution with 40% glycerol on the
side of the drop and wait for crystals to stop dancing. Fish them out swiping
through the side were you have put your solution. Or I have used MPD as
cryoprotectant and freeze them in cold room (4C) its amazing what c
Ahh, yes, the "dancing crystals problem". The good news is alcohols are
really good cryoprotectants as well as excellent precipitants. Shame
their use has fallen out of favor over the years, but I guess as drop
sizes got smaller the evaporation problems got worse and worse.
In my humble opi
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structural biology broadly defined as experimental
