[ccp4bb] Glycoprotein expression strategy

2018-07-11 Thread WENHE ZHONG
Dear Community, A little bit out of topic here. An enzyme we like to use in our assay is commercially available. However, we found some unfavorable activities that probably from the contaminants from this commercial source. We checked the company website and found out this enzyme was purified f

[ccp4bb] Vacancy at Evotec UK (Abingdon)

2018-07-11 Thread Matthias Zebisch
Dear All, There is an opportunity to join the Structural Biology team at Evotec based in the UK as a Senior Scientist (Protein purification). Please see the advert in the link below. Interested candidates should apply directly through our website. Please do not send applications through to me d

[ccp4bb] salt bridges etc..

2018-07-11 Thread Eleanor Dodson
How do people decide on what is a salt bridge within a molecule and how to count them for those Tables? I have been looking at 2z2f - paper claims some score..- But there are several residues in alternate conformation with NZ A to OE1Aand NZ A to OE1B and NZ B to OE1B etc Is that one salt

Re: [ccp4bb] R-flag choose

2018-07-11 Thread Kay Diederichs
On Tue, 10 Jul 2018 22:08:48 +0100, Eleanor Dodson wrote: ... > In general if you have NCS your Free R >values tend to be lower than expected.. >Eleanor > ... and with tNCS, the Rwork / Rfree values tend to be higher than expected. Kay ##

Re: [ccp4bb] Joint CCP4 and ESF-EACBM Newsletter number 31

2018-07-11 Thread Pedro Matias
Hi Tomas, Thanks very much! I also have a printed copy (somewhere) but in this day and age a digital copy is much more convenient! Best, Pedro Às 09:18 de 11/07/2018, Tomas Malinauskas escreveu: > Hi Pedro, > it seems PostScript files (one can easily convert to PDFs) with the > contents are h

Re: [ccp4bb] Joint CCP4 and ESF-EACBM Newsletter number 31

2018-07-11 Thread Harry Powell
Hi I can't see it on the web but I have a hard copy on my bookshelf... Harry -- Dr Harry Powell Chairman of European Crystallographic Association SIG9 (Crystallographic Computing) On 11 Jul 2018, at 09:00, Pedro Matias wrote: > Dear CCP4ers, > > Is there a PDF version of the Joint CCP4 an

Re: [ccp4bb] Joint CCP4 and ESF-EACBM Newsletter number 31

2018-07-11 Thread Tomas Malinauskas
Hi Pedro, it seems PostScript files (one can easily convert to PDFs) with the contents are here: ftp://ftp.ccp4.ac.uk/ccp4/newsletter/nov_94/ Best wishes, Tomas On Wed, Jul 11, 2018 at 9:01 AM Pedro Matias wrote: > Dear CCP4ers, > > Is there a PDF version of the Joint CCP4 and ESF-EACBM Newslett

[ccp4bb] Joint CCP4 and ESF-EACBM Newsletter number 31

2018-07-11 Thread Pedro Matias
Dear CCP4ers, Is there a PDF version of the Joint CCP4 and ESF-EACBM Newsletter number 31 (November 1994)? I can find the summary on the web but not the contents. Thanks in advance, Pedro -- Industry and Medicine Applied Crystallography Macromolecular Crystallography Unit ___