Dear Colleagues,
The June 15th deadline for applications to the CSHL X-ray Methods in Structural
Biology Course to be held later this year, October 15 through October 30 2018,
is rapidly approaching. This is a unique opportunity to become expert in the
use of X-ray crystallography to solve cha
Liuqing Chen,
Everything that has been said seems reasonable, but there are always infinite
possibilities in crystallisation, so it is more a question of priorities. Do
the easy (or quick) things first. If you have buckets of prepared protein then
what you will try first might be different than
Dear All,
There is currently a position available for a Senior/Principle Protein
Production Scientist at Peak Proteins Ltd based in the UK at Alderley
Park near Manchester. Please see the link below for further details. All
applications (CV plus a covering letter) should be sent to
j...@peakp
Dear All,
We have an pen postdoc position in our Small Molecule Research department at
Bayer Crop Science in Monheim close to Cologne. All details can be found in the
job advertisement under the following link:
https://www.karriere.bayer.de/en/job/Postdoc-Protein-Structural-Biology-m-f--SF22513_e
Probably echoing others but if you are looking at these cryogenically, small
changes in the cryoprotectant concentration can have a big effect, similarly pH
tweaking can help. Your conditions seem to be very similar to a commercial
cocktail and a little optimization may go a long way. That said,
Dear all,
I have been informed that the original links will eventually lead to the
application system in the Finnish language, which might not be optimal for
some. Here are links that will allow to apply in English:
post-doc
https://rekry.saima.fi/certiahome/open_job_view.html?did=5600&jc=1&id=
Just a quick note- even if the crystals appear the same (i.e. the same
morphology in a light microscope), that doesn't necessarily mean that they
diffract the same. Did you try putting some of these optimised crystals into an
x-ray beam?
Or as previously suggested, try them at room temperature?
Dear Liuqing,
It could be good to dissolve the crystals to check if the crystallised protein
is still intact. If it is truncated then make a new construct based on
truncation. It could improve the crystal diffraction as it was the case for me.
Best wishes,
Mohinder
--
Hi Liuqing,
1) When you say 8Å diffraction - did you test the crystals at room temperature?
2) Change the construct. Trim loops and termini, (re)move tags, etc.
HTH,
Dave
From: CCP4 bulletin board on behalf of Liuqing Chen
<519198...@163.com>
Sent: 04 June
The point is that *once you have a 3D representation of the RL*, you
can map whatever RS metric you like on that presentation and articulate
its effect on real space. In case of resolution measures this is straight
forward;
where it becomes interesting is at other atrocities and mutilations of
Dear Bernhard and Gerard,
congratulations for nice results that both you (and Gerard team) have been
contributed and published !
Just a nice occasion to remind (and slightly correct Bernhard's message in that
article) that the 'efficient resolution' that we suggested (2013) is NOT a
single g
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