[ccp4bb] Reference for hanging drop crystallization

2018-03-16 Thread Shane Caldwell
Hi ccp4bb, I've been asked to provide a reference for making hanging-drop crystals, and I suppose that I never realized the need to cite the technique specifically. Is there a definitive publication where one could trace the origins of the technique, or a particularly thorough textbook or review?

[ccp4bb] ccp4 release 7.0 update 053

2018-03-16 Thread Charles Ballard
Dear All a further update to the series 7.0 ccp4 release containing * refmac5 - bug fixes and speedup * crank2 - bug fixes * arcimboldo * morda * fragon (linux and osx) * buccaneer * nautilus * ccp4mg All the best Charles ps - next update to contain shelxe and a monomer library update

Re: [ccp4bb] Looking at an EM map..

2018-03-16 Thread Garib Murshudov
Yes. that is how it should be done. if you have a map then you convert them to Fourier coefficients directly using Fourier transformation. Only for some particles density maps are positive everywhere. X-ray particles are one of them. For neutron and electron scattering maps could be negative as

[ccp4bb] Subject: CCP-EM Icknield Workshop for High Resolution Model Building and Refinement - Registration Open

2018-03-16 Thread Tom Burnley
Dear all, We are proud to announce the 2018 Icknield Workshop kindly supported by Instruct. This course is aimed at structural biologists with high resolution EM maps ready for / in the process of model building and refinement. This four day course (1st-4th May) will host some of the leading softw