Dear Cheng,
Chris and Ruud have provided you with the typical interpretation of such a
motif, but you have forgotten to give the CCP4 community the context of this
leucine-repeat helix. Is it amphipathic? Does the protein also have
transmembrane helices (as suggested by the figure provided) w
it's always worth trying optimization, you never know.
also try to get a room-temperature diffraction image of your crystal, only then
will you know if the cryo or freezing didn't damage it. If at RT it diffracts
to high resolution, you will then know that you have to work on the
cryo-condition
Dear All
Morda at ccp4online has been updated.
The structure solution program is improved and database is extended .
The update is also available for existing local Morda installations and can be
installed from command line: change to installation directory (by default
MoRDa_DB) and type ./upd
Assuming that your homology model is that of a dimer, you could put it in a
large unit cell (just add CRYST1 record). The only interface you will get from
pisa will be your dimer interface.
If your homology model is a monomer, then pisa will not help, of course, and
you would need to pred
Absolutely you should optimise! It's impossible to predict crystal behaviour. I
had a 20 A crystal, and I set a new plate in the same reservoir with an
additive screen and got a 3A crystal. It was probably a completely different
crystal to be honest, lattice, etc, but one never knows, you just h
