Hi Yu,
Have you tried using the Additive Screen (e.g. from Hampton Research) on
top of your crystallization condition, and varying the ratio between
protein complex and crystallization solution? From the diffraction pattern,
it looks you are almost there. Salt crystals don't show spots close to th
Some of the high intensity, high resolution spots are possibly from ice.
Diana
**
Diana R. Tomchick
Professor
Departments of Biophysics and Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd.
Rm. ND10.214A
Dallas, TX
I would say definitely not a quasicrystal, just multiple crystals, but the
spots look like salt spots to me, being very sharp and intense. The puzzling
thing is that the low-resolution zone is so populated, which would argue a bit
against salt.
JPK
+
I also would love to see an image….
JPK
+
Jacob Pearson Keller
Research Scientist / Looger Lab
HHMI Janelia Research Campus
19700 Helix Dr, Ashburn, VA 20147
Desk: (571)209-4000 x3159
Cell: (301)592-7004
+
There are programs which are good at indexing patterns from multiply
twinned crystals. Bruker AXS has one, to my knowledge. There may be other
sources. I suggest you try that first before you invoke a quasicrystal
explanation.
James Phillips
On Tue, Feb 13, 2018 at 12:07 PM, Takanori Nakane w
Hi,
"dials.reciprocal_space_viewer" is very useful to identify multiple lattices.
For quasicrystal and modulated crystals, "dials.rs_mapper" is also very
useful.
Best regards,
Takanori Nakane
> Have you tried microseeding of these sphere crystals? It may help to get
> better crystals.
>
>
> Bur
Have you tried microseeding of these sphere crystals? It may help to get better
crystals.
Burak
From: CCP4 bulletin board on behalf of Yu Qiu
Sent: 13 February 2018 15:09:43
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] protein quasicrystals?
Hi,
I have be
Colleagues,
The Spiller lab in the Department of Pharmacology at Vanderbilt University is
seeking a Research Assistant to assist Dr. Professor Spiller in his laboratory.
The Spiller lab is a structural biology lab focused on infectious disease and
the host’s immune response. The job requires a m
I am very interested in this topic, and have found incommensurately
modulated crystals and few examples of possible protein quasicrystals.
Do you have any images you could share?
On Tue, Feb 13, 2018 at 9:09 AM, Yu Qiu wrote:
> Hi,
>
>
>
> I have been trying to crystallize a protein complex and k
Hi,
I have been trying to crystallize a protein complex and keep getting sphere
shape crystals. The diffraction is around 3 angstrom, but looks like multiple
lattices. I am wondering if it could be a quasi crystal? Is there anyone has
such experience?
Thanks,
Yu
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