Hello everyone!
In which condition can we add ligand/substrate directly to the expression
medium for overexpression protein? One protein of i crystallized is a enzyme
that catalyze sterols substrate, as we know the solubility of sterols is very
poor, and the Km of the enzyme towards sterols a
Seems like you are looking for something like DARA.
https://dara.embl-hamburg.de/
-Amin.
On Sat, 9 Dec 2017 at 3:21 AM, Vands wrote:
> Hi All,
>Is there any Web server available where I can input my SAXS
> profile and it will give all closely matched PDB structures from the all
> av
Hi Vandna,
A typical approach would be to generate different models (experimental or
otherwise, if X-ray structure it could be "completed" further by modeling
missing loops / side-chains) of your sequence to calculate a SAXS profile,
and then compare / fit those to experimental SAXS profile. Some
Yes thank you very much .. I used it two years back and just blanked out.
:)
-Vandna
On Fri, Dec 8, 2017 at 3:55 PM, amin sagar wrote:
> Seems like you are looking for something like DARA.
>
> https://dara.embl-hamburg.de/
>
> -Amin.
>
> On Sat, 9 Dec 2017 at 3:21 AM, Vands wrote:
>
>> Hi All,
Hi All,
Is there any Web server available where I can input my SAXS
profile and it will give all closely matched PDB structures from the all
available PDB.
--
Vandna Kukshal
Postdoctral Research Associate
Dept. Biochemistry and Molecular Biophysics
Washington University School of Medic
Thanks Eleanor, John and Nigel for your tips. Actually it seems to be a problem
of geometry restraints.
Nigel, let me edit the .def file as John recommended and then I'll contact you.
Best regards
Gerardo Andrés Libreros
Laboratório de Biologia Estrutural Aplicada
Universidade de São Paulo
Sã
Hello,
only a test of the biological function of mutants will tell whether
your interface is an artefact or not.
It is very well possible that an alanine mutations increases binding;
you have to inspect the interface carefully whether there were for
example buried hydrogen donors or flexible
