Hello,
I had probelms this week with
1) Pymol 2.0 where clicking on the different legacy plugins (except
lighting) caused shut down of Pymol 2.0
2) CCP4 old style aborted upon re-start-up (which I do far too rarely...).
Upon restarting the mac,it appeared that several "Updates" of I do not
k
It is also possible that it co-purifies via a nickel IMAC column purification
step. The affinity of many E. coli proteins for such columns is well known.
Diana
**
Diana R. Tomchick
Professor
Departments of Biophysics and Biochemistry
University of
Dear All,
To add my voice to those who wrote crystallization artifacts happen - we
just witnessed one today. A postdoc from another lab tried crystallizing
a protein for months. Today, during data collection from his poorly
diffracting crystals, one of our guys (Dr. Porebski) scaled the data a
Hi all
Since my recent upgrade to High Sierra, XQuartz (most recent version 2.7.11 -
fresh installation) does not longer behave properly. Essentially, X11 does not
seem to understand touchpad actions (for example cannot close X11 windows,
cannot drag them around, etc.). This problems also affec
If you cannot trust the surface of your protein, perhaps you should not look at
the the potential on the surface. Instead you can look at the field around your
protein. This is less precise, but also less sensitive to local errors. If you
want to know how your peptide finds your protein, this is
I agree with Roger Rowlett, try a room temp capillary mount. Also ,what is
the magnification of the microscope/TV system you are using to center these
tiny crystals? Centering the loop is not enough.
James Phillips
On Fri, Dec 1, 2017 at 8:48 AM, Thomas, Leonard M. wrote:
> Looking a bit close
These are not easy questions to answer. Certainly atoms,
particularly ones that are charged, even with fractional charges, have a
strong effect on the ESP. If you delete them because you don't know
exactly where they are you will get a different answer than if you put
them in in some reasonabl
Hi
I am calculating the Electrostatic Potential of my protein. But there were
few flexible region with high B-factor and I deleted that part of the
protein and then recalculated it. But there I can see a big change.As I
have a structure in the presence and the absence of the peptide and the
these
Dear all,
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description for the job. Should you have any question regarding the position,
please directly contact Dr. Kim at c...@bcm.edu.
An NIH funded postdoctoral research scientist position is available at Dr.
Choel
Looking a bit closer are you sure the crystal is protein? I see a couple of
spots which could indicate a small molecule of some variety.
Len
On Nov 30, 2017, at 3:04 PM, Geny mailto:gzh...@gmail.com>>
wrote:
Dear EveryOne,
I recently tried to collect the diffraction data, but unluckil
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