On behalf of Professor Neil Ranson (Director of EM, Astbury BioStructure
Laboratory)
Dear All,
A new 3-year BBSRC-funded position in my lab is now available. The project is
to work on high resolution structures of new plant viruses using the state of
the art cryo-EM facilities with the Astbur
Satvik - if you attach your data processing logs I can tell you what to
look out for - too abstract to do without a concrete example to discuss.
Eleanor
On 20 September 2017 at 14:00, wrote:
> Dear Satvik,
>
>
>
> An R/Rfree of 0.29/0.35 after one round of automatic model building
> indicates th
Dear Satvik,
An R/Rfree of 0.29/0.35 after one round of automatic model building indicates
that your solution is correct. You can proceed with refinement and rebuilding.
You can take either the monomer-based or dimer-based solution, it does not
matter. Personally I would take the monomer-based
Hello,
Thanks all for your valuable suggestions. Two things that stuck me were "one
gets to know if the space group is correct only after solving the
structure" and "to try all possible space groups until one lands up with
the correct solution".
I ran Phaser with "all alternative space groups". T
Hi Rashi,
Rather than use the mtz file from EDS you should use he mmCIF reflection file
from the PDB directly and convert it. If the authors deposited the test set,
you can keep it this way. Otherwise you need to select a new test set.
Note that if you want to optimize an existing PDB entry, the
Dear Tony
The only requirements we have for numbering is that every residue must
be unique when using a combination of residue name (to handle
microheterogeneity), residue number, insertion code and chain ID.
During curation we will try to map your protein sequence to UniProt -
please see th
