[ccp4bb] Post-Doc position in Structural Biology, Bourne Lab @ University of Oklahoma, Norman OK

2017-09-13 Thread Christina Bourne
Postdoctoral Position in Structure-Function Studies of Toxin-Antitoxin Systemsat the University of Oklahoma, Norman OK TheBourne lab at the University of Oklahoma seeks to recruit a postdoctoralscientist with experience in Biochemistry and/or MacromolecularCrystallography and interests in bac

[ccp4bb] ice build up during collection using 4 axis goniometer

2017-09-13 Thread Annette Herta Erbse
Hi Pius, I just wanted to say that Rigaku is absolutely helping me trouble shoot this. They are really good in providing support. I just posted this here in case someone had seen this before and had a solution that I am missing because I don't have a huge amount of experience with this yet. I

Re: [ccp4bb] ice build up during collection using 4 axis goniometer

2017-09-13 Thread Edward Snell
Hi Annette, We did some studies way back when that profiled the temperature signature of a cryostream as a function of distance using an infrared camera ago (JSR 2007, 14, 109-115). The original study looked at beam heating from the X-ray beam but in the process we noticed that the stream can h

Re: [ccp4bb] ice build up during collection using 4 axis goniometer

2017-09-13 Thread Francis Reyes
I've had this problem with the four circle goniometers. I imagine your setup looks similar to https://www.med.upenn.edu/biocbiop/jf/bsbcore/images/X-ray_Rigaku_MicroMax-007.jpg ? At high chi values, I would also observe a 'tail' of ice off my pins. As you mentioned in the manual, it's because

Re: [ccp4bb] ice build up during collection using 4 axis goniometer

2017-09-13 Thread Sanishvili, Ruslan
Hi Annette, I do not have any experience with the particular set up you are using, but just from general considerations, how about: 1. If you have control over humidity in the room, reduce it as much as you can during "icy" data collections 2. In another e-mail you mentioned you use coppe

[ccp4bb] ice build up during collection using 4 axis goniometer

2017-09-13 Thread Annette Herta Erbse
Hi Scott and Henry , Thanks you for the fat response. I am using copper pins. What is interesting for me is that it really only happens at certain geometries. As long as I don't move chi I can collect for days, if I set chi to let's say 400 and Omega brings the pin to move so that it forms a sma

[ccp4bb] ice build up during collection using 4 axis goniometer

2017-09-13 Thread Annette Herta Erbse
Dear All, We have a Rigaku XtalLab with a MM003 generator, a 4 axis goniometer and an Oxford 700 cryostream. I have been running into the problem that if I make full use of the 4 axis goniometer in the collection strategy I run into geometries where the angles between the pin/Xtal and the O

[ccp4bb] Postdoc Position Available in Structural and Functional Study of Macromolecular Complexes In Transcription And Repair at UCSD

2017-09-13 Thread Dong Wang
Please help to post following postdoc recruitment ad. Many thanks. A postdoc position, supported by NIH funding, is available in the lab of Dong Wang at University of California, San Diego. Our research focuses on the interplay between two fundamental processes: transcription and

[ccp4bb] Fwd: Granada Crystallization Boxes?

2017-09-13 Thread Gloria Borgstahl
Dear friends, I am resending this post just in case any of you have Granada Crystallation Boxes (GCBs) in your lab supplies that you do not need. The NASA protein crystal growth community could really use them. Please see my message below. Thank you, Gloria -- Forwarded message ---

[ccp4bb] Postdoc position to study the structural biology of ubiquitin ligases at Queen Mary University of London

2017-09-13 Thread Benjamin Stieglitz
Dear all, A postdoctoral position is available in the lab of Ben Stieglitz at the School of Biological and Chemical Sciences at Queen Mary University of London. The position will focus on the characterisation of the molecular mechanism by which bacterial E3 ligases subvert the eukaryotic ubiqui