Dear Frank,
I may see in the attached pic several nucleation points and a considerable
amount of microcrystals. Based to my knowledge decreasing the concentration
of the precipitant and/or the protein concentration would be a reasonable
approach to refine the initial hits.
By checking the diagram
Actually, you should try /increasing/ the protein concentration - a
lot. But be prepared to drop the precipitant concentration to almost
nothing (1 or 2% isn't "low").
To understand why, look at the phase diagram and what we assume about
vapour diffusion. (Which I'm assuming is what you're d
Dear Patrick,
You may reduce the protein concentation, as well.
Another option could be the *streak seeding* by exploiting the drop of your
initial condition.
Good luck,
V.T.
On Mon, Jul 10, 2017 at 7:17 PM, Patrick Shaw Stewart wrote:
>
> Microseed them into two or three random screens.
>
>
Absolutely agree
Eleanor
On 11 July 2017 at 22:06, Keller, Jacob wrote:
> Still seems to me that the resolution could and should be pushed a little
> further at least—CC1/2 is still high, completeness is good, I/sigma also is
> good. Why not extend a little further, say to where one of these val
Still seems to me that the resolution could and should be pushed a little
further at least—CC1/2 is still high, completeness is good, I/sigma also is
good. Why not extend a little further, say to where one of these values gets
too low? Might improve the maps a bit.
JPK
From: CCP4 bulletin boar
That makes sense then - you have solved it am sure.
Eleanor
On 11 July 2017 at 20:36, Koromyslova, Anna <
a.koromysl...@dkfz-heidelberg.de> wrote:
> Sorry for the confusion, NCS was found only in a dataset where the cell
> dimensions were twice bigger regardless of the sp, and with a dimer as a
>
Sorry for the confusion, NCS was found only in a dataset where the cell
dimensions were twice bigger regardless of the sp, and with a dimer as a
solution. The solvent content was the same.
Peak Distance Vector
73.3% 143.8Å: FRAC +0.000 +0.000 -0.500 (ORTH -0.00.0 -143.8).
There was no
Triana is no longer making or selling them
On Tue, Jul 11, 2017 at 1:39 PM, Diana Tomchick <
diana.tomch...@utsouthwestern.edu> wrote:
> You can continue to buy new ones from Tirana Science & Technology, which
> is a Spanish company.
>
> http://www.trianatech.com/index.php?option=com_content&;
>
Sorry, that’s Triana Science & Technology (darn automatic spell-check!).
Diana
**
Diana R. Tomchick
Professor
Departments of Biophysics and Biochemistry
University of Texas Southwestern Medical Center
5323 Harry Hines Blvd.
Rm. ND10.214A
Dallas, TX
You can continue to buy new ones from Tirana Science & Technology, which is a
Spanish company.
http://www.trianatech.com/index.php?option=com_content&view=article&id=65&Itemid=88&lang=en
Diana
**
Diana R. Tomchick
Professor
Departments of Biophysi
So SG could be P31 2 2
What is the height of NCS vector v origin?
Eleanor
Look at hklview to see hk i sections. Obviously all l = odd will be weak.
On 11 July 2017 at 19:12, Koromyslova, Anna <
a.koromysl...@dkfz-heidelberg.de> wrote:
> Dear Eleanor,
>
>
>
> NCS translation vector = 0 0 -0
Dear Eleanor,
NCS translation vector = 0 0 -0.5 in the final sp P6222, and in P622 or C121.
All had the same solution.
The protein tends to form a homodimer and if I use P1 space group molecular
replacement can find several dimers, there is no translational ncs found during
MR, but still solven
I have recently found out that these are no longer being manufactured or
sold commercially. But, as fortune has it, we have just been funded to fly
some large quartz capillaries crystallization experimente up to the
International Space Station for neutron crystallography. Our experimental
design
Anna,
Eleanor is raising very important question: if you have NCS then there should
be another similar entity in the asu. Have you detected off origin peak while
using your final P6222 or lower sg?
From: Koromyslova, Anna [mailto:a.koromysl...@dkfz-heidelberg.de]
Sent: Tuesday, July 11, 2017 1
Dear Vaheh and Phil,
Sorry, I was a bit misleading – antibody fragment I used is a nanobody (VHH,
15kDa). I was worried about the cell content because when I started the pdb
deposition, there was a warning that solvent content is expected to be below
80%, so I thought maybe I missed a correct s
This is very strange . If you have a large non- crystallographic
translation vector you would expect either to have two molecules in the
asymmetric unit or your one molecule must have two very similar domains?
What is the n-c translation vector?
Could you have assigned too high symmetry ? SG maybe
Похоже, что вы уже решили структуру. Почему вас беспокоит плотность ваших
кристаллов? Кристаллы бывают разные.
First of all Fab by itself is already almost 50 kDa, so complex with antigen
should be more than 50 kDa. Because you already solved the structure calculate
the molecular mass based on
Hello Anna
You've already found the correct number of molecules in the asymmetric
unit. 21% Rwork is a quite respectable value for a structure at this
resolution, and while 80% solvent is a relatively rare occurrence it's
not unprecedented (a couple of years back I did one at 3.0Å with 75%
s
Dear CCP4 members,
I am working on a structure of a protein in complex with an antibody fragment
(approx. 50kDa together). Molecular replacement with closely related proteins
always comes up with one complex in the asymmetric unit, although MW of protein
to which Matthews applies is 125kDa and
dear petr,
several phytochrome structures seem to represent mixed-state crystals.
best,
jon
-Ursprüngliche Nachricht-
Von: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] Im Auftrag von Petr
Kolenko
Gesendet: Dienstag, 11. Juli 2017 16:00
An: CCP4BB@JISCMAIL.AC.UK
Betreff: [ccp4bb] Inq
Dear colleagues,
We are working on a paper, where we want to discuss our crystal
structure. We have determined structure of non-active state first (much
easier). Than we tried to convert the protein into active state by
soaking (direct crystallization not possible). We have observed more
than
Dear all,
The Brown lab at Harvard Medical School has an opening for a postdoctoral
scientist.
The lab uses the latest developments in cryo-EM to understand biological
processes at the molecular level (e.g. Nature 524, 493–496 and Science 346,
718–722). The project will involve investigating t
Dear Andreas,
did you try the option '-t' to make SHELXE try harder, or other building
related options (take a look at the SHELX web page for talks and
presentations).
Can you make use of the phases without model building? (sfall)
Since you seem to get a PDB-file, could you use the coordinates
Hello again,
Back in 2001 people still remembered the difference between Rsym and Rmerge. 16
years later people seem to have forgotten. I will try to dig even more ancient
references… Archaeology is the name of the game.
http://www.ysbl.york.ac.uk/ccp4bb/2001/msg00383.html
Cheers,
Fred.
From
Hello Frederic. Interesting. Have you got some reference on this to share?
James
Dr James Foadi PhD Diamond Light Source Ltd. Diamond House Harwell Science and
Innovation Campus Didcot Oxfordshire OX11 0DE United Kingdom office email:
james.fo...@diamond.ac.uk alternative email: j.fo...@imperial
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