These kind of "crystals" are very common with DDM, I guess they could be called
spherulites. Sometimes they develop one or a few straight edges after a while.
I don't think these are DDM crystals as DDM is very soluble, but
protein-detergent crystals that are dominated by weak detergent micelle
Tom,
I doubt that they are DDM crystals, as all the pure
BETA-alkyl-glucosides/maltosides are not known to crystallize. Also they are a
bitch to crystallize under normal conditions of detergent purification (hence
their high cost and the existence of less pure “solubilization” grade
products)
Hi, all.
I have obtained some crystals (see the imgur link) from my crystal trials of a
membrane protein and I'm concerned that they may be detergent, DDM.
The drop condition is:
0.2M NaCl
0.1M HEPES @ pH 7.0
22% PEG500 MME
Protein sample contains (GF running buffer):
0.02M Tris
0.1M NaCl
0.
Sent from my Bell Samsung device.
Original message
From: Clemens Vonrhein
Date: 2017-06-16 4:43 AM (GMT-06:00)
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Problem with Mg2+ binding site refinement
Hi,
On Fri, Jun 16, 2017 at 10:47:20AM +0530, Prem Prakash wrote:
> Hi
Hi Clemens,
> -Original Message-
> From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
> Clemens Vonrhein
> Sent: Friday, June 16, 2017 12:42
> To: CCP4BB@JISCMAIL.AC.UK
> Subject: Re: [ccp4bb] Problem with Mg2+ binding site refinement
>
> Hi,
>
> On Fri, Jun 16, 2017 a
Hi,
On Fri, Jun 16, 2017 at 10:47:20AM +0530, Prem Prakash wrote:
> Hi Mubinur,
> I got the same situation while refining my protein complex with Lanthanum
> ion complex, reducing the occupancy while refining solved the problem.
That might have not been the whole story through. You still want to
Dear All,
Thank you all for you valuable suggestions. I have checked the pdb and found Mg
is there. Now I will try according to your suggestions.
- Mubinur
On 15.6.2017, at 20.47, Pavel Afonine
mailto:pafon...@gmail.com>> wrote:
Hi Mubinur,
try without "metal restraints" and see if that hel
