Congratulations!
I think you are now looking for additional crystallographic and
non-crystallographic symmetry, because finding 40 particles in arbitrary
positions and orientations is going to be brutal.
I wouldn’t take the cell and point group assignment from XDS at face value.
Rather I thin
Use Zanuda to see whether the space group is actually a higher one—looks like a
and c axes are pretty similar, and beta might be 120, suggesting a threefold.
Otherwise it’s a pretty large beta. I wonder what the largest beta ever seen in
the pdb is?
JPK
From: CCP4 bulletin board [mailto:CCP4B
The real SG is most likely not P1. I asked Phaser to search for 4
molecules, it found 3. Thanks for the advice, I will try it out.
Best,
Roger
On Wed, Apr 26, 2017 at 2:50 AM, wrote:
> Hi Roger,
>
>
>
> First, sigma is a relative measure. If sigma is very low, e.g. since your
> map contains 80%
I searched for 4 copies, but Phaser only found 3. It's possible there is an
additional copy of the molecule in the ASU
On Wed, Apr 26, 2017 at 2:40 AM, Randy Read wrote:
> Dear Roger,
>
> You didn’t mention this, but did you search for 2 copies (or an even
> number of copies) and, if so, did Pha
Greetings all,
I am having trouble with a data set and would like to know if somebody can
help. I'm working with a protein of approximately 50 kDa, which I have
successfully crystallized. The crystals diffracted at a resolution of 3,65
angstroms and upon initial processing using XDS i obtained th
Hi,
Site http://ccp4wiki.org/ is not working.
E-mail of the responsible person (as fetched by whois service) c...@dl.ac.uk
is not accessible.
Can someone inform responsible person about this issue?
P.S.: It seems we read too much.
--
Eugene Osipov
Junior Research Scientist
Laboratory of Enzyme En
Dear Liuqing Chen,
for this purpose I designed an experiment to check using SDS-PAGE if
your HA is bound to your protein or not, this way you don't have to
worry about migration on native-PAGE.
It requires the use of HA that bind covalently to Cys, like HgAc, MMA,
HgCl2, AuCl4, PtCl4, PtCl6, o
