Thanks Eleanor,
If I understand right, there is just 1 TFZ. TFZ== means "Translation
Function Z-score equivalent, only calculated for the top solution after
refinement (or for the number of top files specified by TOPFILES)" so there
could be many TFZ==.
Twinning analysis attached below:
TWINNING
Hi Sutapa,
With regards to insect cell expression, how do you know it wasn't expressed?
You didn't see a band by coomassie staining or by western?
If by staining, I'd recommend to just try expressing a limited amount through
infection of a few 150mm dishes of your favorite cell line (I use Hig
It’s p3221. Re-process your data forcing this space group, rebuild, and refine
twin domains. Just do it—you won’t regret it!
Jacob
From: Eleanor Dodson [mailto:eleanor.dod...@york.ac.uk]
Sent: Friday, April 14, 2017 3:19 PM
To: Keller, Jacob
Cc: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Refma
Hi,
apart from the possibilities of conformational flexibility affected by crystal
packing, just wondering if this mutation is supposed to actually cause an
increase or decrease in the corresponding activity (outside the context of a
crystal)? k(cat) and K(M) measured in solution would help her
maybe attach your data processing log file..
e
On 14 April 2017 at 20:10, Eleanor Dodson wrote:
> That twin factor list means the apparent crystal symmetry must be P6/mmm.
>
> You say you only have 2 molecules in the asymmetric unit of P32,therefor
> there must only be one in SGs P32 21 P32 12
That twin factor list means the apparent crystal symmetry must be P6/mmm.
You say you only have 2 molecules in the asymmetric unit of P32,therefor
there must only be one in SGs P32 21 P32 12
So I dont understand why you have PHASER results like this:
SOLU SET RFZ=4.4 TFZ=7.7 PAK=0 LLG=55 TFZ==
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As I mentioned off-list, it would be helpful to know how many types of search
models you are searching with—how many different molecules are in the complex?
It’s hard to interpret MR results otherwise.
Also, since the higher-symmetry SG works in MR, you should try to refine the
model in that SG
Thanks Eleanor, I tried MR for P32 21 and P32 12.
SG P3221: SOLU SET RFZ=5.3 TFZ=8.8 PAK=0 LLG=121 TFZ==11.2 LLG=944
TFZ==29.2 PAK=0 LLG=944 TFZ==29.2
SOLU SPAC P 32 2 1
SG P3212:
Solution #1 annotation (history):
SOLU SET RFZ=4.4 TFZ=7.7 PAK=0 LLG=55 TFZ==9.6 LLG=350 TFZ==20.5
PAK=0
Dear Elizabeth,
The High-Throughput Crystallization Screening Center and the Hauptman-Woodward
Medical Research Institute has been operating for over a decade with
considerable success. There are comprehensive details at http://getacrystal.org
but basically your samples are screened against a
Hi Elizabeth,
HWI, University of Buffalo, NY offers high-throughout crystallization
screen. See the link for more information:
http://hwi.buffalo.edu/science/high-throughput-crystallization-center/
Good Luck
Partha
On Fri, Apr 14, 2017 at 9:06 AM Elizabeth Diaz
wrote:
> All,
>
> I am currently
Dear Pierre
Daniel has raised a valid point either you use the same column for the
purification of mutant and wt. It happens in our case as well as we see the
activity of the inactive mutant but it is supposed to be caused by the
using the same column. Just compare the enzyme activity before perfor
All,
I am currently attempting to crystallize a peptide/protein complex and am
wanting to know about robotic screening services that you would recommend.
We have done some in house conditions with little luck, and want to broaden
our search, but that would require going externally for screening. D
I think in his case the WT does not perform the reaction in the crystal, but
the mutant does.
Haven't heard of examples of this before, but perhaps the WT needs "something"
to do the reaction, like a conformational change impossible in the crystals, a
co-factor or something else, perhaps even im
Hi Colleagues!
I hope you can come to our symposium on Understanding Biology through Structure
in Santa Fe this May 13-17!
The Symposium will emphasize interactions between junior and senior
researchers. We hope that this will present a special opportunity for junior
researchers to present p
USP not UPS. Sorry about the auto spelling correction feature of my smart (or
not smart!) phone.
-- Original message--From:
xinhuang...@yahoo.com<0317accc4341-dmarc-requ...@jiscmail.ac.uk>Date: Fri,
Apr 14, 2017 7:34 AMTo: CCP4BB@JISCMAIL.AC.UK;Cc: Subject:[ccp4bb] Co-crystal
struct
Hi, all.
Could you let me know if there is a co-crystal structure of any UPS in complex
with a small molecule inhibitor? I cannot seem to find any.
Thanks.
First - four way twinning is possible but pretty rare for macromolecules
Pointless gives a very useful table of the CC agreement for each possible
symmetry operator individually.
In this case with only two molecules in the asymmetric unit you you could
only have a higher symmetry SG as
P32 21 P32
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