Re: [ccp4bb] FW: [ccp4bb] Using a codon-optimised gene to improve protein solubility

Hi Sutapa, Slow expression did work in my case. I had to induce with 100uM IPTG, and grow cells at 10C for 48 hours. It left at least 50% of expressed protein in soluble fraction. Best, Swarna On Wed, Apr 5, 2017 at 1:52 AM, AJV wrote: > Hi Sutapa, > > The input from other commenters has been

Re: [ccp4bb] Voltage-Gated Structures

Dear Jacob, 5VB2 and 5VB8 on the PDB show open and closed forms of a sodium ion voltage-gated channel from Arcobacter butzleri (https://www.ncbi.nlm.nih.gov/pubmed/?term=28348242). Hope this helps. [http://www.ncbi.nlm.nih.gov/coreutils/img/pubmed256blue.png]