Dear CCP4bb members,
I have a dataset of 3A, twinned data (twin fraction 0.15) set of space
group P6222, I solved the structure by phaser (TFZ>10, LLG>1000) with space
group P32 and build model to Rfree/R 30% 26%. I then tried twin refinement
in Refmac, after I tried amplitude based refine the Rf
Dear crystallographers -
I've been trying without success to find a suitable system to test a method for
predicting change in binding energy (delta-delta-G) due to mutations of
protein-protein complexes that result in a change in net charge. In particular
I'm looking for a system with the fol
Hi All,
Just share my experience, Windows 10 works for pymol 3D under Quadro M4000
with Nvidia 3D kit (no need to connect the 3 pin MINI DIN, Quadro M4000
does not have 3 pin Mini DIN connections). I use Asus 24 inch 3D monitor,
connect the monitor with display port to display port of Quadro M4000
I'll add another favorite to that one (below.) I especially enjoy the tracking
of the intensities of the Bragg *rods* along their lengths, showing how the 2D
lattice only chops reciprocal space in two dimensions, leaving the third
dimension continuous.
JPK
Nature. 1975 Sep 4;257(5521):28-32.
T
Hi Rich,
I'm not sure but I imagine it would also apply to single span proteins.
I have never encountered a helical membrane protein that could be boiled w/o
aggregating, even very stable ones. Of course, a more elaborate sample
preparation involving boiling, sonication and urea as mentioned b
Dear ccp4bb,
1. Why would you need a precipitant to crystallize a protein?
The principle of salting-in/salting-out means that as you remove the salt
that
keeps a protein soluble, the protein will tend to come out of solution and
given
the right conditions, crystallize.
2. From the solubilit
Dear Phoebe,
I am sure Jakob is aware of this publication and the impressive work as much
as I am. The two of us were discussing electron crystallography (not
microscopy!) of 3D crystals, not of 2D crystals. The first such structure was
deposited at the PDB by Tamir Gonen no earlier than 2013.
How have you assigned the Free R set? If there is twinning you need to make
sure that the related reflections have the same FreeR assignment.
This is now standard in the new FreeR software written by Ville, and called
from GUI2, but it used to require some jiggery poky to achieve.
Next question -
Several postdoc positions are immediately available in Prof. Ian A.
Wilson’s Lab of Antibody Structure at Shanghai Institute for Advanced
Immunochemical Studies (SIAIS), ShanghaiTech University (
http://siais.shanghaitech.edu.cn/eng/research7.asp), China. SIAIS is an
innovative research institute d
