Hi Tomas,
Thanks. I think this one is directed at a C-terminal his6-tag. I'm not sure
it'll work with an N-terminal tag.
Elias
From: Tomas Malinauskas
Sent: Sunday, January 8, 2017 5:39:59 PM
To: Fernandez, Elias J
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4b
Dear Fernandez et al.,
maybe this anti-His-tag antibody could be useful: PDB ID 1KTR, its
sequence is in the paper
https://www.ncbi.nlm.nih.gov/pubmed/?term=12054774
Hope that helps,
Tomas
On Sun, Jan 8, 2017 at 9:24 PM, Fernandez, Elias J wrote:
> Dear All,
>
> Is anyone aware of a recombinant s
Besides my private reply, a few suggestions that may be relevant to the
broader audience:
1) for the purposes of this email I will pretend that relevant patents do
not matter (of course they do but it's too complex to get into details)
2) there are quite a few antibodies out there directed against
Dear All,
Is anyone aware of a recombinant system that produces an N-terminal,
hexa-histidine-directed Fv antibody fragment that can be used for structural
studies (crystallography, SAXS, cryo-EM)?
Best regards,
Elias
