Re: [ccp4bb] Extreme off-topic on protein expression

2016-11-12 Thread Artem Evdokimov
Impossible to say without knowing details of the particular protein. Could be trivial or could be impossible. Artem www.harkerbio.com "See us at PSDI 2016 in Malmo" (some messages are just what they seem) On Nov 13, 2016 1:04 AM, "Israel Sanchez" wrote: > Dear all, > > for reasons difficult to

Re: [ccp4bb] Extreme off-topic on protein expression

2016-11-12 Thread Keller, Jacob
Well, there’s circular permutation, but that’s somewhat different. If the arrangement of the domains is important, this also may not work. And the linkers should be important as well. JPK From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Israel Sanchez Sent: Saturday, Novem

[ccp4bb] Extreme off-topic on protein expression

2016-11-12 Thread Israel Sanchez
Dear all, for reasons difficult to explain in a brief email, I am interested in flipping the order of domains in a poli-peptide in bacteria. The scenario would be a multi-domain protein, let's say containing 3 domains DI, DII and DIII, which are arranged N-ter to C-ter: DI-DII-DIII. The protein

Re: [ccp4bb] 3D viewing room options

2016-11-12 Thread Gert Vriend
Hia Xavier, For about 10 years already we use a simple solution consisting of a cheap computer with two graphics cards, a beamer from the game industry that can project stereo, and simple liquid crystal shutter glasses. This gives us projection on the wall (just a painted wall, nothing special

Re: [ccp4bb] 3D viewing room options

2016-11-12 Thread Brian Smith
Hi Xavier, Any LG "Cinema 3D" capable TV should give you good passive stereo for the kind of thing you need and definitely work with PyMol and COOT under LINUX with a low end Nvidia Quadro (not NVS) card such as the K620 using the NVidia drivers. Sadly LG seem to have dropped 3D support from th