[ccp4bb] How to fit BioSAXS shape to the Structure

Dear all, I am new to saxs and I get the model by saxs data and I have a structure. I want to fit the structure to the shape but I can just open them in PyMol. Can any one teach me to fit them? Best, Weifei

Re: [ccp4bb] Interesting DNA contamination

Stepwise addition to 1% PEI (polyethylenimine) following cell lysis (before dialysis) should do the trick. --paul On 06/25/2015 05:23 PM, Pramod Kumar wrote Dear all Sorry for off topic and lengthy post, but I came across a very unique DNA contamination during one membrane protein purificati

Re: [ccp4bb] Interesting DNA contamination

I think the more interesting questions are: should one want to disrupt such a tight interaction? Wouldn’t the structure of the protein bound to DNA be more interesting than the protein alone? Why not try to crystallize the complex and show how the protein binds? Sometimes you should just run with

Re: [ccp4bb] Interesting DNA contamination

Several different approaches may help you to separate DNA from protein including; 1) When bound to the nickel column, wash the protein/beads with 1-2M NaCl or 1-2M KCl for a few hours to overnight. Increase ionic strength should disrupt protein-DNA interactions. 2) Use a low concentration of ur

[ccp4bb] Postdoc position in Structural Biology and Biochemistry at Sussex University

Postdoctoral Research Fellow (Ref 256) School of Life Sciences, University of Sussex Full time, fixed term for 3 years Salary range: Grade 7 starting at £31,342 and rising to £37,394 per annum Closing date for applications: 25th July, 2015 Expected start date: 1st September 2015 Description Appli

Re: [ccp4bb] Interesting DNA contamination

Dear Pramod Kumar, if the DNA binds to the protein, wouldn't this affect the interpretation of the Agarose gel? 500 bases should result in a distinct shift during gel filtration. Do you observe this? While waiting for suggestions, you might set up crystallisation trials just in case? Best wishe

Re: [ccp4bb] Interesting DNA contamination

pramod Do a spin after bacterial cell breaking at 8K to get rid of a nuclear pellet Also there is a nuclease preparation available called benzonase it is most effective when you also add magnesium in your buffer so in your homogenization buffer add magnesium and should avoid any EDTA Pius On Thu,

[ccp4bb] Interesting DNA contamination

Dear all Sorry for off topic and lengthy post, but I came across a very unique DNA contamination during one membrane protein purification (a microbial external environment sensor/response protein) Already done * DNAse used as stranded protocol during cell break. * Membrane extraction to Ni-Affin

Re: [ccp4bb] Request

Dear Neeraj, Do you mean the H3C protease ( trade name: PreScission protease provided by GE)? This protease can specifically cleavage the sequence encode by plasmid pGEX-6p, which possess the GST tag. Best wishes! Lu Zuokun -- 卢作焜 南开大学新生物站A202 Lu Zuokun, Ph.D. Candidate College of

[ccp4bb] PhD position in protein biochemistry and structural biology

We are currently looking for a highly motivated Ph.D. student for our laboratory at the Dept. of Biochemistry at University Bayreuth, Germany. Our work involves biochemical and structural studies on cellular signalling systems with relevance to aging mechanisms or disease. The PhD project will comp