Might be worth trying to see if your protein will still crystallize in a
mixture of tris and TAPS buffer? The pKa of the latter is very close to tris,
but goes in the opposite direction with temperature - a roughly 3:2 TAPS:tris
mix should have minimal pH change on freezing.
Tristan Croll
L
This paper might be helpful.
1. J Struct Biol. 2014 Nov;188(2):102-6. doi: 10.1016/j.jsb.2014.09.011. Epub
2014
Oct 5.
Efficient cryoprotection of macromolecular crystals using vapor diffusion of
volatile alcohols.
Farley C, Juers DH.
Author information:
(1)Department of Physics, Whitman Colle
Hi All,
We have gotten some very nicely formed crystals out of a couple of different
volatile solvents recently. Besides looking for something easier to work in
does anybody have any tips on handling crystals from these types of solvents.
It is very hard to loop a crystal while it is doing th
Does anyone have experience with Tris buffer in cryo protectants? I would
expect the pH of the cryosolution to increase a lot during flash freezing
which could perhaps destroy the diffraction. I rarely use Tris for
crystallization but the current protein really prefers Tris. I would
appreciate any
Hello,
how does one specify C-terminal amidation of a peptide for REFMAC
refinement? I intended to prepare link restraints between the C-terminal
amino acid residue and a residue "NH2". But JLIGAND reports "Code 'NH2'
does not exist in the library". On my system, $CLIB/data/monomers/n/NH2.cif
exist
In case it’s of interest (for pub discussions of structural biology, explaining
yourself to your parents, or perhaps teaching…), I thought I’d point people to
a couple of pieces on the Guardian Science blog this week about the problem of
seeing yourself as a molecular entity:
This is my take o
