Re: [ccp4bb] collection strategy to collect the intersection with a reference set.

2015-02-24 Thread Kay Diederichs
Hi Francis, XDS (XPLAN) just prints tables. Instead of looking in these for the range that maximizes the total completeness, you could just minimize the total completeness. This would maximize the intersection. HTH, Kay On Tue, 24 Feb 2015 11:37:05 -0500, Francis Reyes wrote: >Hi all, > >I

Re: [ccp4bb] hydrophilic protein going to aggregate

2015-02-24 Thread shivendra singh
Hi Anita, Try to lower the ph of binding buffer if your protein allows also you may lower the concentration of salt like nacl if adding into the buffer also try to reduce the imidazole concentration of your eluted fraction gradually by performing serial dialysis before SEC. Alternatively, you can t

[ccp4bb] hydrophilic protein going to aggregate

2015-02-24 Thread Anita P
Hello Crystallographers, I am trying to express and purify a soluble domain of a membrane protein for crystallization. The amino acid content is as below Ala (A) 12 13.8% Arg (R) 10 11.5% Asn (N) 2 2.3% Asp (D) 8 9.2% Cys (C) 1 1.1% Gln (Q) 1 1.1% Glu (E) 4 4.6% Gly (G) 16 18.4% His (H) 3 3.4% Il

[ccp4bb] Post-doctoral position

2015-02-24 Thread Belinda Bullard
University of York, Department of Biology Post-doctoral Research Associate The molecular basis of stretch activation in muscle A post-doctoral position is available to work on the stretch activation of muscle, using insect flight muscle (Lethocerus and Drosophila) as a model system. The aim of

[ccp4bb] Postdoc position in structural biology of multiprotein complexes, SGC University of Oxford

2015-02-24 Thread Wyatt W. Yue
Dear all, We seek a post-doctoral scientist with strong background in structural biology and protein-protein interactions, to drive an industry-funded project studying the molecular mechanism of iron-sulphur cluster assembly and associated genetic disorders. http://www.ndm.ox.ac.uk/current-job

Re: [ccp4bb] RMSD of dimers

2015-02-24 Thread Thomas Holder
Hi Dan, gaps/insertions should be no problem for PyMOL's rms_cur command, as long as chain identifiers match (and all other atomic identifiers!). See http://pymolwiki.org/index.php/Fit for a description of the matchmaker argument. Cheers, Thomas On 24 Feb 2015, at 16:30, D Bonsor wrote: >

[ccp4bb] RMSD of dimers

2015-02-24 Thread D Bonsor
I have a family of homodimers (denoted A1B1, A2B2, A3B3...) which I have superimposed using Chain A. Several programs will produce the RMSD of Chain A2, A3, A4... to Chain A1. However, I would like to know the RMSDs of Chain B2, B3, B4... to Chain B1 when I have superimposed the structures relat

Re: [ccp4bb] error message in xds

2015-02-24 Thread Boaz Shaanan
Hi, As I understand it you don't have enough spots for indexing. You may want to try and increase the number of frames from which spots are taken for indexing (SPOT_RANGE), maybe even to the maximum. You can have a look at COLSPOT.LP (the printed output of the spot collecting process) and SPO

[ccp4bb] error message in xds

2015-02-24 Thread jeorgemarley thomas
Dear All, I am getting an error message after indexing the data in XDS as " !!! ERROR !!! DIMENSION OF DIFFERENCE VECTOR SET < 2" , please suggest what wrong has happened and what could be done for this, initially the message was insufficient number of spots accepted was there, then I change the r

[ccp4bb] Postdoctoral position

2015-02-24 Thread Belinda Bullard
Please could you advertise the attached postdoc position?   BBSRC-postdoc.doc Description: application/applefile BBSRC-postdoc.doc Description: Binary data Dr. Belinda BullardDept. of BiologyUniversity of YorkYork YO10 5DDUKTel 44(0)1904 328823FAX: 44(0)1904 328825email: bb...@york.ac.uk

[ccp4bb] collection strategy to collect the intersection with a reference set.

2015-02-24 Thread Francis Reyes
Hi all, I'm on the lookout for a prediction program that'll tell me the best collection angles that match the reflections of a reference set, as opposed to the the reflections that'll complete a reference set. It seems that mosflm and XDS prescribe the angles for completion rather than the in

[ccp4bb] Postdoc in Membrane Protein Structural Biology

2015-02-24 Thread F V
POSTDOCTORAL POSITION IN MEMBRANE PROTEIN STRUCTURAL BIOLOGY A NIH funded postdoctoral position is available immediately in the laboratory of Dr. Francis Valiyaveetil, Department of Physiology and Pharmacology, Oregon Health and Science University in Portland, Oregon. The Valiyaveetil group inv