Hi Francis,
XDS (XPLAN) just prints tables. Instead of looking in these for the range that
maximizes the total completeness, you could just minimize the total
completeness. This would maximize the intersection.
HTH,
Kay
On Tue, 24 Feb 2015 11:37:05 -0500, Francis Reyes
wrote:
>Hi all,
>
>I
Hi Anita,
Try to lower the ph of binding buffer if your protein allows also you may
lower the concentration of salt like nacl if adding into the buffer also
try to reduce the imidazole concentration of your eluted fraction gradually
by performing serial dialysis before SEC. Alternatively, you can t
Hello Crystallographers,
I am trying to express and purify a soluble domain of a membrane protein
for crystallization. The amino acid content is as below
Ala (A) 12 13.8% Arg (R) 10 11.5% Asn (N) 2 2.3% Asp (D) 8 9.2% Cys (C) 1
1.1% Gln (Q) 1 1.1% Glu (E) 4 4.6% Gly (G) 16 18.4% His (H) 3 3.4% Il
University of York, Department of Biology
Post-doctoral Research Associate
The molecular basis of stretch activation in muscle
A post-doctoral position is available to work on the stretch
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Dear all,
We seek a post-doctoral scientist with strong background in structural
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Hi Dan,
gaps/insertions should be no problem for PyMOL's rms_cur command, as long as
chain identifiers match (and all other atomic identifiers!).
See http://pymolwiki.org/index.php/Fit for a description of the matchmaker
argument.
Cheers,
Thomas
On 24 Feb 2015, at 16:30, D Bonsor wrote:
>
I have a family of homodimers (denoted A1B1, A2B2, A3B3...) which I have
superimposed using Chain A. Several programs will produce the RMSD of Chain A2,
A3, A4... to Chain A1. However, I would like to know the RMSDs of Chain B2, B3,
B4... to Chain B1 when I have superimposed the structures relat
Hi,
As I understand it you don't have enough spots for indexing. You may want to try and increase the number of frames from which spots are taken for indexing (SPOT_RANGE), maybe even to the maximum. You can have a look at COLSPOT.LP (the printed output of
the spot collecting process) and SPO
Dear All,
I am getting an error message after indexing the data in XDS as " !!! ERROR
!!! DIMENSION OF DIFFERENCE VECTOR SET < 2" , please suggest what wrong has
happened and what could be done for this, initially the message was
insufficient number of spots accepted was there, then I change the
r
Please could you advertise the attached postdoc position?
BBSRC-postdoc.doc
Description: application/applefile
BBSRC-postdoc.doc
Description: Binary data
Dr. Belinda BullardDept. of BiologyUniversity of YorkYork YO10 5DDUKTel 44(0)1904 328823FAX: 44(0)1904 328825email: bb...@york.ac.uk
Hi all,
I'm on the lookout for a prediction program that'll tell me the best collection
angles that match the reflections of a reference set, as opposed to the the
reflections that'll complete a reference set.
It seems that mosflm and XDS prescribe the angles for completion rather than
the in
POSTDOCTORAL POSITION IN MEMBRANE PROTEIN STRUCTURAL BIOLOGY
A NIH funded postdoctoral position is available immediately in the laboratory
of Dr. Francis Valiyaveetil, Department of Physiology and Pharmacology, Oregon
Health and Science University in Portland, Oregon. The Valiyaveetil group
inv
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