When trying to adjust the "chi" angles of a dT in coot 0.8.1, the methyl and
its H's (which I called C5M to make phenix happy) rotate with the sugar,
producing a rather base bizarre geometry.
Yes it can. Ethidium stains DNA by intercalating between base pairs, so if
most of your DNA is tightly bound by protein the ethidium may not be able to
wiggle in there. We've seen that happen with Mu transposase - DNA complexes.
If that's the problem, soak your gel in SDS plus EtBr and you sh
Hello all.
I'd like to understand what it is I'm looking at when I use Coot's density
fit analysis tool. I recognize that there was a post related to this
topic on the Coot bb a while ago --the discussion was on how to interpret
the red-ness or green-ness of the density fit plot (
https://www.mail-
Dear all,
Thank you so much for all the replies and suggestions in such a short period of
time since I posted my questions! I am slightly overwhelmed by the number of
things that I could try in the near/medium future! One thing that I am
recommended a lot is TaBr. It seems that this compound is
On 19-2-2015 1:38, Smith Lee wrote:
Dear All,
It often finds for the Ramachandran favored determined by Coot, MolProbity
regards as Ramachandran outliers. There are earlier posts regards Coot and
MolProbity has different database for the determination of the Ramachandran
plots. Then will you p
