Re: [ccp4bb] CCP4BB Digest - 13 Aug 2014 to 14 Aug 2014 (#2014-220)

Unsubscribe Sent via the Samsung GALAXY S®4, an AT&T 4G LTE smartphone Original message From: CCP4BB automatic digest system Date:08/14/2014 6:00 PM (GMT-06:00) To: CCP4BB@JISCMAIL.AC.UK Subject: CCP4BB Digest - 13 Aug 2014 to 14 Aug 2014 (#2014-220) There are 17 messages

[ccp4bb]

http://indaproje.com/iletisim/Cath.php

Re: [ccp4bb] dynapro DLS cuvettes

At hellma-analytics dot com, the closest match seems to be catalog number 176-353-15-40, but, that's an HPLC flow cell. Daniel Anderson wrote: Hello, Gloria and everybody, I'm typing most of this reply from memory. When I tried to buy one, my recollection was that it was available from Hellm

Re: [ccp4bb] dynapro DLS cuvettes

Hi, A crazy solution may be to make a cuvette with a 3D printer. I'm not sure if the available resins are transparent to DLS. Best wishes, Reza Reza Khayat, PhD Assistant Professor The City College of New York Department of Chemistry, MR-1135 160 Convent Avenue New York, NY 10031 Tel. (212) 6

Re: [ccp4bb] dynapro DLS cuvettes

Hello, Gloria and everybody, I'm typing most of this reply from memory. When I tried to buy one, my recollection was that it was available from Hellma, but I couldn't (and still can't) find my Hellma paper catalog, and for some reason I did not find the Hellma web site when I wanted to buy a

Re: [ccp4bb] CC-half value ??

Exactly. Aimless will give you suggested resolution cutoffs based on CC 1/2 in the log file. Roger Rowlett On Aug 14, 2014 5:04 PM, "conan仙人指路" wrote: > Hi Faisal, > > CC-half standard is valuable in evaluating the cut-off of highest > resolution. Sometimes even if I/sigI is close to 1 and com

[ccp4bb] dynapro DLS cuvettes

Does any one know of a source of these cuvettes? Protein Solution doesn't exist anymore and Wyatt no longer has these.

Re: [ccp4bb] monomer/dimer protein

Hi Todd, Do you mean reversible or irreversible dimers? If the binding is reversible, then once crystal growth starts, Le Chatelier's principle should take over, pull the equilibrium toward monomer, and you should see minimal impact of dimer on your "monomer crystals". The same would be true in r

Re: [ccp4bb] CC-half value ??

Hi Faisal, CC-half standard is valuable in evaluating the cut-off of highest resolution. Sometimes even if I/sigI is close to 1 and completeness is not as high, if CC-half is still significant, it may be worth incorporate the extra high-res shell data and extend the resolution. Again, if only

[ccp4bb] CC-half value ??

Dear all How CC-half value of a data set determines the maximum resolution limit during data processing ?? Although much we know about the Rsym and I/Isig values of the highest resolution shell while processing the data, what are the parameters we need to check related to CC-half values ?? -- Re

Re: [ccp4bb] desiring untouched ligand

Remie, Look at the following webpage: http://www.ysbl.york.ac.uk/refmac/data/refmac_news.html The harmonic restraints need to go into a "keyword" file that is uploaded to the "refmac keyword field" field in the GUI. Philip On Thu, Aug 14, 2014 at 11:11 AM, Remie Fawaz-Touma wrote: > Thank y

Re: [ccp4bb] desiring untouched ligand

Thank you Dr. Murshudov for the information. But please I still need help. In restrained refinement, I could not find where to enter the residues I want to restrict from moving much. I was only able to find that in rigid body refinement, so I tried it and got a higher R factor than the initial f

[ccp4bb] Kinase postdoc positions available at SGC, Oxford University

Two postdoc positions are available at the SGC labs at the University of Oxford to work on the structure and inhibition of human protein kinases. The first is a Wellcome Trust funded project (CAMSEED) working with Stefan Knapp to develop inhibitors against the protein kinase CaMK1D in breast can

Re: [ccp4bb] Twinning in space group Pc

Dear Kristof, Apart from things pointed out, a possibility to consider would also be the presence of two different crystal types--of different compounds--in the same reaction vessel. Best regards, Navdeep --- On Wed, Aug 13, 2014 at 11:00:18AM +0200, Kristof Van Hecke wrote: > Dear, > > I’m

Re: [ccp4bb] Difference between different b factors values

Dear Faisal, at 2.6A resolution I would not call 23A^2 and 24A^2 'a difference', rather a fluctuation. Parameters are not totally independent from each other and not from experimental issues. It most likely also make a difference which program you use. Some program has a upper cut-off of 100-200 (

[ccp4bb] AW: [ccp4bb] Difference between different b factors values

Dear Faisal, The Wilson B-factor is the B-factor of the diffraction data and is calculated by the data processing software. The B-factor of the atoms is produced by the refinement program. Ideally the Wilson B (B-factor of the data) and average B of all atoms (B-factor of the model) should be v

[ccp4bb] CC-half value

Dear all How CC-half value of a data set determines the resolution limit for the data processing ?? Although much we know about the Rsym and I/Isig values of the highest resolution shell while processing the data, what are the parameters we need to check related to CC-half values ?? -- Regards

[ccp4bb] Difference between different b factors values

Dear all I request you to please tell me the difference between wilson B-factor and average B, all atoms ?? I have two structures one native at 2A resolution, having mean b factor of 24 and the other a complex structure of the same protein with a ligand (at 2.6A resolution) has mean b factor of 23