Dear All,
I have been trying to answer the following questions:
1) In the context of optical imaging, how is a protein crystallography
reconstruction validated? How meaningful is this perspective of protein
crystallography (optical imaging)?
2) In transition to single particles (single unit cells
Hi Rhys,
Have you tried quick back-soaks into solutions lacking the heavy atoms? This
can reduce radiation decay caused by absorption of X-rays by overabundant heavy
atoms.
Best,
Chris
> On Jul 27, 2014, at 4:48 PM, RHYS GRINTER
> wrote:
>
> Hi All,
>
> I thought I might put a question t
How about merging multiple crystals together a la Wayne Hendrickson's most
recent papers?
JPK
-Original Message-
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Boaz
Shaanan
Sent: Sunday, July 27, 2014 6:34 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] Heavy A
Hi,
While some useful phase information could perhaps be extracted from the data
you currently have, I'd suggest to try the method of quick soak described
here: Acta Cryst. (2002). D58, 1092-1098 rather than the long soak that you
tried. I guess that you also tried complete new screens for th
Hi All,
I thought I might put a question to the community, with the hope of getting
some tips of the best way to proceed with my heavy atom phasing problem.
I'm working on solving the structure of an integral beta-barrel membrane
protein of approximately 100 kDa. I've crystallised protein, growi
