[ccp4bb] cryoprotection condition

Dear CCP4 Thank you for your suggestions Best Regards Rana

Re: [ccp4bb] Bper lysis reagent recipe

B-PER is basically two components: 1. ~0.5% octyl-beta-thioglucopyranoside 2. 25 mM TRIS pH 7.5 A while ago when I priced out the detergent separately the bulk price came up to be about the same as the price of pre-made B-PER from Pierce. So I did not bother to check it out any further. Of course

[ccp4bb] Bper lysis reagent recipe

Lovely CCP4BB Members, Hopefully not too far off topic: It appears that our sonicator is, ironically, bust. Whilst I wait for a new one, and/or some B-PER reagent, does anybody have a home made detergent lysis reagent (something like B-PER) recipe? Only E. coli to bust open (for now). Many Tha

[ccp4bb] off-topic mutated enzymes

Dear Colleagues, Apologies for the off-topic question: We are developing an approach for ‘Protein Repair’ to be utilized for the enhancement of activity defective proteins. Ideal candidates for us are enzymes that can be isolated for in-vitro activity assays, and for which single amino acid su