F1 ATPase. Got some Nobel glamour too.
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From: "Aaron Thompson"
Date: Tue, Jan 28, 2014 01:10
Subject: [ccp4bb] Examples of multiple ASU copies with different conformations
To:
The structure of kappa opioid receptor fused with T4 lysoz
Hi Shane,
One of my favorite examples of large structural differences between molecules
in the asymmetric unit have is the crystal structure of the L1 ribozyme (PDB ID
2oiu). The Q and P chains have two stems which crudely co-axially stack and a
third stem which appears 180 degrees in the oppos
The structure of kappa opioid receptor fused with T4 lysozyme (4DJH)
contains two copies in the ASU – each copy displays a different orientation
between the receptor and lysozyme.
On Mon, Jan 27, 2014 at 10:08 AM, Shane Caldwell wrote:
> Hi ccp4bb,
>
> I'm putting together a talk for some peer
Dear Faisal,
The PDB_REDO server returns a .pdb file with residual B-factors after TLS,
to submit a model to the PDB it has to have total B-factors. To get those
you can run the CCP4 program TLSANL. The program can read the TLS
information straight from the .pdb file, but if you have an older vers
Dear all
I have solved a structure of a protein at 2.1A..the R and free R is 21
and 26..for the betterment of its refinement statistics i submitted
the coordinate and structure factor file to we based server "PDB_REDO"
which improved the R and Rfree to 18 and 23, (which probably does
through TLS r
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Hi ccp4bb,
I'm putting together a talk for some peers that highlights strengths and
weaknesses of structural models for the outsider. For one point, I'd like
to find some examples of proteins that show very different conformations
between different copies in the ASU. One example I know of is c-Abl
Hi Chen,
I had a similar condition before (reservoir solution with 10% dioxane) and an
oil-based cryoprotectant (66.5% w/w paraton-N, 28.5% paraffin oil and 5%
glycerol) worked for me. Please check this paper out:
http://scripts.iucr.org/cgi-bin/paper?wd5052
In addition, have you tried cryoprot
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Hi Fellows,
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