Another thing to try when cryoprotecting any crystals grown at room temp using
a volatile precipitant is to first transfer the crystal tray to 4 degrees, then
quickly add a cold aliquot of a viscous cryoprotectant to the drop. Evaporation
is considerably slower at 4 degrees, and I prefer to use
Dear Mark,
Thank you so much for your reply. Interestingly, after several days I
posted this question, I found both my previous crystals and precipitant
completely dissolved, but instead much larger crystals start to appear.
However, most of them are intergrown together, only few are single. And I
Chen,
Dioxane is not easy to work with, exactly for the reasons you describe.
There is one thing you did not mention, which I know to be an additional issue:
the quality of the dioxane. I do not know if you need good quality (whatever
that is) but it is a fact that crystallization works with
Hi all,
I thought the bulletin board might be interested to know that one of the
contributors on Popular Science is featuring a webcomic series on X-ray
crystallography. The first post went up yesterday and can be found here (
http://www.popsci.com/blog-network/boxplot/x-tallographers ). If nothin
Dear BB,
If you are, or know, a keen, talented, motivated student looking for a PhD,
then there are several opportunities within the Faculty of Biological Sciences
at the University of Leeds.
One project involves the use of stapled peptides to investigate inhibition of
protein-protein interacti
We are inviting applications for a postdoctoral position immediately available
in Dr. Jiang’s laboratory at Drug Discovery Division of University of
Wisconsin-Madison School of Pharmacy. An ideal candidate will undertake a
project that applies multidisciplinary approaches (e.g., enzymology, mass
You dont say whether the unit cells are the same?
In cases like this I sbmit both sets of coordinates to PISA - to get the
preferred biological unit and check whether each structure has similar
contacts.
PISA is distributed with CCP4 but the version at the EBI pdb gives prettier
results!
Eleanor
Dear all,
This is the final announcement for the Neutrons in Biology and Biotechnology
meeting which will be held at the Institut Laue Langevin on the 19th - 21st
February 2014.
The programme has an exciting list of speakers and concludes with an open
ILL/PSB colloquium from Tim Hunt (Nobel
Dear Colleagues,
Description for a postdoctoral position in the lab of Dr Anne-Marie Krachler,
University of Birmingham, UK;
Post is available from 1 March 2014
Postdoctoral Research Fellow (Structural Biology)
Applications are invited for a highly motivated post-doctoral researcher with
expertis
Dear all,
I don't know how closely this relates to what James pointed at, but
regarding the aspect of NCS information usage in the heavy-atom
finding/refinement stage, I would like to mention two programs of my
knowledgethat are able to detect NCS in a set of putative heavy atom
sites froma subst
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