Hi Acoot:
Can your protein form some kind of dynamic self oligomers? When you test by
using the gel-filtration column, if the peak is symmetry? Sometime if the
target protein can have week self interaction, you can observe a tailed peak.
If the protein can have a strong self interaction, maybe
Dear Brett,
We have seen this behaviour several times for different adenovirus fibre head
proteins and don't really have an explanation for it. We have always set the
peaks up separately when we had enough protein.
For this purification, as you don't have enough protein to pool them
separately,
Hi Acoot,
since they behave differently on IEX, they are different - you would introduce
heterogeneity into your crystallization setup, which usually is not a good idea
for crystallization in general.
Are you using Benzonucleases by any chance in your preparation and if not, that
may explain th
Dear All,
When I purified my protein by ion exchange chromatography for crystallization,
there were several peaks containing the target protein as analyzed by SDS-PAGE.
All these peaks have the same MW as determined by gel filtration coupled MALLS.
For crystallization purpose, can I merge the
On Fri, 13 Dec 2013 19:44:44 +, D Bonsor wrote:
Dear D,
I agree with Tim and Jürgen that
a) the map after Phaser, and before refinement is the most unbiased and should
be used for sequence assignment.
b) there may be a sequence register shift error that is responsible for the
high R-value
