Dear Jens, Careina
this is not really an *advantage*, but rather a *convenience*. You can still
use big crystals if you'd like to, but as they usually never survive more than
one shot (few femtosec), you'd need a lot of these bigger crystals to collect a
full data.
And yes, I would also highly
Hi,
I am facing a problem in refining one of my structures. With every
refinement the Rwork is going down. However, the Rfree is stuck and is
not going down with refinement. I have already solved the structure
without the co-factor bound. This structure is with the co-factor
bound. Can anyone sugg
Dear all,
Does anyone know how to calculate the cell parameter of the 2-D protein crystal?
The map of the crystal can be found in this blog:
http://dingding830106.blog.163.com/blog/static/351191702013111014448546/
Thanks for your time!
Best!
Wei
Dear developers,
We have been doing the "local" scaling with scala of multiple
wavelengths for some time. We scaled one dataset collected remotely from
the absorption edge, and input this to scala as a reference as one batch
without any problems. With the latest release of CCP4, though, we get
th
Careina,
If your target is interesting enough, try to reproduce the small
crystals in batch and apply for FELS time. Small crystals are actually
an advantage there.
Cheers,
Jens
On Wed, 2013-12-04 at 21:41 -0800, Careina Edgooms wrote:
> Hi all
>
>
> Any advice on how to get bigger crystals
Hello,
We have a postdoctoral position available in 2014 studying membrane proteins
involved in the innate immune system. This position is based in Professor
Jennifer Martin's laboratory at the Institute for Molecular Bioscience in
Brisbane, Australia. The applicant should hold a PhD in structu
Phil,
How about Alchemist from Rigaku automation
Please consider Alchemist for your next purchase
http://www.rigaku.com/products/automation/alchemistht
Padayatti PS
On Mon, Dec 9, 2013 at 10:46 AM, Phil Bourne wrote:
> The Biomek 2000 is capable of setting up screens. It can handle viscous
> l
The Biomek 2000 is capable of setting up screens. It can handle viscous
liquids if the liquid level sensing tools are combined with very slow
pipetting. My experience with Biomek 2000 systems is that it is not quicker
to prepare anything with them. Their advantage comes in reproducibility and
consi
Dear All
I just installed CCP4-6.4 and all the subsequent updates. Now MrBump failed
with the following error message:
The program run with command: /usr/local/ccp4-6.4.0/bin/ccp4-python -u
/usr/local/ccp4-6.4.0/bin/mrbump HKLIN
/home/jie/Structures/local/se-p26c8newn6/ccp4_6.3/high_x4c.mtz SEQI
Dear All,
Apologies for the off-topic post, but I think there might be a number of people
subscribed to this list who also use ITC and would find this useful:
I am pleased to announce the immediate availability of the
software program NITPIC. You may download it from
http://biophysics.swmed.
Hello,
Molecular Dimensions Ltd (Newmarket, England) is seeking an enthusiastic
sales-oriented individual to help drive our marketing effort and support our
distributors.
The rôle will be based in Newmarket and includes providing training and support
for our distributor network and assisting a
Dear all,
does anyone have experience in using a Biomek 2000 liquid-handling
system for preparing screens? How does it perform with viscous solutions
- e.g. PEGs? How difficult / time consuming are programming and maintenance?
Your help is highly appreciated.
Toni Meinhart
Hi,
We used to allow degenerate solutions (i.e. ones in which only the orientation
is known but not the position, or in which the orientation and position within
a plane perpendicular to a rotation axes are known), because these can be
scored by the same likelihood target used for the rotation
another option might be the age of the screen perhaps ?
very silly suggestion/question: your pH meter is calibrated before you make
your measurements right ?
If you have a nano-pH probe (anything that can be added to a 96well reservoir
well) you could test what the final pH of your composition i
Hello, Nasir,
What concentration of your ammonium sulphate stock solution? Do your
solution remains immiscible without ammonium sulphate?May be addition of
concentrated solution of ammonium sulphate to PEG MME could cause phase
separation? Anyway, try to heat your solution a bit as recommended
Hello,
Can somebody tell me how the Phaser degenerate function can be used with
MR_BTF?
My script is as follows:
#my_phaser_run.com
phaser << eof
TITLe BTF_RUN
MODE MR_BTF
HKLIn a.mtz
LABIn F=Fobs SIGF=Sigma
ENSEmble mol1 PDB a.pdb IDENtity 100 # this is model structure not
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