[ccp4bb] small crystals

Hi all Any advice on how to get bigger crystals from conditions that give showers of tiny crystals? I am getting small pretty looking individual crystals but they are too small and they don't seem to grow. In fact, in some instances if left for a couple of days they actually dissolve. I have fi

Re: [ccp4bb] Can Mathew's coefficient tell about a complex

If either of the two protein structures has been determined/deposited, I would check if your unit cell matched one of them.   Kris F. Tesh, Ph. D. Department of Biology and Biochemistry University of Houston From: "Tanner, John J." To: CCP4BB@JISCMAIL.AC.UK Se

Re: [ccp4bb] Can Mathew's coefficient tell about a complex

First of all, there are two Ts and no apostrophe in Matthews. The method is based on the following paper, which is worth reading. It is one of the first examples, possibly the first, of structural bioinformatics. J Mol Biol. 1968 Apr 28;33(2):491-7. Solvent content of protein crystals. Matthew

Re: [ccp4bb] Can Mathew's coefficient tell about a complex

Looks like 1 or 2 protein units per ASU. Two seems most likely, but I have had a bunch of crystals with 67% solvent, too, so 1 is not out of the question if less probable. So try both with your favorite MR method and see what happens. Crystal packing in the MR solution will tell all. _

[ccp4bb] Can Mathew's coefficient tell about a complex

Dear All, I crystallized a complex of two proteins and got x-ray data. However I dont know if I have got complex or just one of the protein has crystallized. Can I check it by mathew's coefficient? If yes how? One of my protein is 30 kDa nad other one is 12 kDa. Actually I calculated Mathew's c

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[ccp4bb] Fwd: [3dem] Cryo-EM 3D Image Analysis Symposium 2014

I am forwarding the meeting announcement below for your possible interest. From: Dorit Hanein We are pleased to announce the first International Conference on Image Analysis in Three-dimensional Cryo-EM, to be held at Granlibakken Conference Center, Lake Tahoe, California, March 12 to March 1

[ccp4bb] REGISTRATION TO THE CCP4 STUDY WEEKEND CLOSES - Information and scientific programme

One week to register - registration for the CCP4 study weekend is closing by 13th December. CCP4 study weekend, Nottingham, Saturday 4th and Sunday 5th January 2014 More than half of the deposited structures in the PDB are oligomeric, and about three quarters are annotated with ligands. This pr

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Dear All, I have a PhD position funded by a BBSRC DTP studentship commencing on October 2014. If you have any enthusiastic final year undgraduate students looking for a PhD to work on the structure of membrane proteins, please pass them this email. The studentship will take place as follows: 1

[ccp4bb] Follow-up: Phaser output in QtRView under Windows XP

Hi again, Following up on my mail earlier this morning, I installed CCP4 in a virtual Windows XP machine on my Mac. When I run Phaser in Windows the QtRView output is lacking the graphical section, as described for the course computers, but I can open the log file generated in the Mac OS X vers

Re: [ccp4bb] TRUNCATE & CTRUNCATE issues.

Hi Robbie, I contacted Charles Ballard a few weeks ago on the very same issue and he has efficiently rolled out a fix for it in the latest CCP4 update :-) Update ID: 6.4.0-001 Date: 25.10.13 Cheers, Jon On 4 December 2013 09:23, Robbie Joosten wrote: > Hi Ian, > > >> This probably doesn't affe

Re: [ccp4bb] TRUNCATE & CTRUNCATE issues.

Robbie That sounds more like a programming error: the issues I've identified are purely numerical and won't affect the program flow. Cheers -- Ian On 4 December 2013 09:23, Robbie Joosten wrote: > Hi Ian, > > > > This probably doesn't affect the MTZ file that comes out of it > > but it will

Re: [ccp4bb] TRUNCATE & CTRUNCATE issues.

Hi Ian, > This probably doesn't affect the MTZ file that comes out of it > but it will affect the statistics of the twinning tests. I'd be interested to see if this solves the cases where ctruncate goes into an infinite loop during the twinning tests. Cheers, Robbie Date: Sat, 30 Nov 2013 15

[ccp4bb] Phaser output in QtRView under Windows XP

Hi, I need (reluctantly!) to install CCP4 on 20 fairly ancient Windows XP machines for a course. I have installed CCP4 on a central server with network-mounted drive and applied all the updates. CCP4 is then runnable on the individual computers. However when I run a Phaser job the output in QtR