Hi Matthias
Thanks for the info about self-SLIC. You wrote "This is because the T4 DNA
polymerase treatment removes the newly synthesized strand in your mutated
region (wich is your SLIC homology region) to have two 5' overhangs for
annealing." Rather than "newly" don't you mean existing or
> May I ask a question?
>
This is a catch-22 situation. If you ask permission to ask a question,
you are already asking a question. Answering this with no would
probably create a wormhole. I'd also like to see a bulletin board where
asking questions requires separate permission.
> When we pre
Dear all,
I am a new starter in the membrane protein field and working with the
cell free expression system. As the really small amount of protein I
could get in hand, I got somehow stuck in the quantification of the
protein yield. so Is there any suggestion of any methods or reagents
suitable for
