Re: [ccp4bb] examples of applied struct bio?

2013-09-16 Thread Jrh
Dear Frank, I would only add to Randy's excellent suggestions a commendation of Max Perutz's book " Is Science necessary" http://www.amazon.com/Is-Science-Necessary-Essays-Scientists/dp/0192861182 It includes global longevity and food production issues and improvements. Of course these are wider

Re: [ccp4bb] off topic: Fluorescence labeled protein, neutral at pH=7

2013-09-16 Thread Appu kumar
Deal Xiang, You can think of GFP/YFP protein attached with any protein would be good for your experiment. These can be easily cloned and expressed. Alternatively one more approach is mentioned below. Chose a protein which has only one cysteine, that too at the surface then you can think of labellin

[ccp4bb] off topic: Fluorescence labeled protein, neutral at pH=7

2013-09-16 Thread 李翔
Hi everyone, I want to get some fluorescence labeled protein for my experiment. I wish it is under 40K Da and have isoelectric point of 7 or slightly lower. Do you have any suggestions on the commercially available protein please? Thanks very much for your help! Sincerely, Xiang -- Li Xiang De

Re: [ccp4bb] Crystallization condition for trimeric protein

2013-09-16 Thread Vandna Kukshal
HI debashish , These crystal might be spherulites. You can try reducing protein concentration in this situation and Microseeding will be the best idea for this. You should crush these crystals by using seed bead or needles and do either seeding with full screen or dilution o

Re: [ccp4bb] examples of applied struct bio?

2013-09-16 Thread Randy Read
Hi Frank, How long of an incubation period between structure, insight and application are you willing to accept? Here are a few possibilities, leaning towards longer incubation times. 1. Structure of DNA -> necessary for understanding of replication, translation, sequencing, etc: contributed

[ccp4bb] examples of applied struct bio?

2013-09-16 Thread Frank von Delft
Hello, I need stuff for a lecture, so I figured I'd best crowd-source it from the best forum on the intertubes: Anybody know some examples of where structural biology threw up insight(s) that led to very significant practical improvements in some public health approach or industrial process or

[ccp4bb] Summary: video of crystal pick-up

2013-09-16 Thread Frank von Delft
What a sensational instant response last week to my request for mounting movies - thanks Here's the list of links (not all verified, I have low bandwidth): * https://www.youtube.com/watch?v=QcsaWowulDM (Dave Hall, Swastik Phulera, Victor Bolanos-Garcia, Rajan Sreekanth) * https://www.

Re: [ccp4bb] What kind of reflection data to deposit to PDB

2013-09-16 Thread Gerard DVD Kleywegt
Dear all, At present, unmerged data cannot be handled properly as part of a PDB deposition. One reason for this is that changes to the mmCIF/PDBx data model will be required (at the moment, hkl must be unique within the reflection data, which is logical for merged data but precludes handling o

Re: [ccp4bb] video of crystal pick-up through microscope?

2013-09-16 Thread Benini Stefano (P)
And when handling liquid nitrogen don't forget to get protections against frost bite (goggles, cryogloves) S. -Original Message- From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Swastik Phulera Sent: Friday, 13 September, 2013 15:08 To: CCP4BB@JISCMAIL.AC.UK Sub

[ccp4bb] Looking for RHOMBIX users

2013-09-16 Thread ipk
dear colleagues, we are about to revive two rhombix plate hotels that have not been used in a while. since thermo is not offereing service anymore, this will be quite an interesting task, but we do have some help and i think we should be able to manage. current users seem to still have support t